{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:16:12Z","timestamp":1761488172211,"version":"build-2065373602"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,12,19]],"date-time":"2008-12-19T00:00:00Z","timestamp":1229644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Main inborn errors of metabolism diagnosable through uracil (Ura) analysis and the therapeutic monitoring of toxic 5\u2010fluorouracil (5FU) in dihydro pyrimidine dehydrogenase (DPD) deficient patients require a sensitive, reproducible, selective and accurate method. In this work, an artificial receptor in the format of molecularly imprinted polymer (MIP) brush \u2018grafted to\u2019 the surface of sol\u2013gel immobilized on cost\u2010effective homemade solid\u2010phase microextraction (SPME) fibers, individually imprinted with either of Ura and 5FU, was used in combination with a voltammetric sensor duly modified with the same MIP. This combination provided up to 10\u2010 and 8.4\u2010fold preconcentrations of Ura and 5FU, respectively, which was more than sufficient for achieving stringent detection limits in the primitive diagnosis of uracil disorders and fluoropyrimidine toxicity in DPD\u2010deficient patients. The proposed method permits the assessment of Ura and 5FU plasma concentrations with detection limits pf 0.0245 and 0.0484\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup> (RSD\u00a0=\u00a01.0\u20132.5%, S\/N\u00a0=\u00a03), respectively, without any problems of non\u2010specific false\u2010positives and cross\u2010reactivities in complicated matrices of biological samples. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1145","type":"journal-article","created":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T21:38:09Z","timestamp":1230154689000},"page":"499-509","source":"Crossref","is-referenced-by-count":22,"title":["Ultratrace analysis of uracil and 5\u2010fluorouracil by molecularly imprinted polymer brushes grafted to silylated solid\u2010phase microextraction fiber in combination with complementary molecularly imprinted polymer\u2010based sensor"],"prefix":"10.1002","volume":"23","author":[{"given":"Bhim Bali","family":"Prasad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khushaboo","family":"Tiwari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meenakshi","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piyush Sindhu","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit Kumar","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shrinkhala","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.01.043"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470281895.ch1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00142-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.02.046"},{"key":"e_1_2_1_6_1","first-page":"11","article-title":"Molecularly imprinted polymers (MIPs) against uracils: functional monomer design, monomer\u2010template interactions in solution and MIP performance in chromatography","volume":"723","author":"Hall AJ","year":"2002","journal-title":"Material Research Society Symposium Proceedings"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(98)00062-2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma0341869"},{"key":"e_1_2_1_9_1","first-page":"COLL","article-title":"2\u2010D molecular imprinting of proteins via polymer brush approach. 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