{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:14:33Z","timestamp":1760645673564,"version":"build-2065373602"},"reference-count":6,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,3,10]],"date-time":"2009-03-10T00:00:00Z","timestamp":1236643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A rapid and sensitive liquid chromatography\u2013tandem mass spectrometric method (LC\u2010MS\/MS) for the determination of bromotetrandrine in rat plasma has been developed and applied to pharmacokinetic study in Sprague\u2013Dawley (SD) rats after a single oral administration. Sample preparation involves a liquid\u2013liquid extraction with <jats:italic>n<\/jats:italic>\u2010hexane\u2013dichlormethane (65:35, containing 1% 2\u2010propanol isopropyl alcohol, v\/v). Bromotetrandrine and brodimoprim (internal standard, IS) were well separated by LC with a Dikma C<jats:sub>18<\/jats:sub> column using methanol\u2013ammonium formate aqueous solution (20\u00a0m<jats:sc>m<\/jats:sc>) containing 0.5% formic acid (60:40, v\/v) as mobile phase. Detection was performed on a triple quadrupole mass spectrometer in multiple reaction monitoring mode. The ionization was optimized using ESI(+) and selectivity was achieved using MS\/MS analysis, <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 703.0 \u2192 461.0 and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 339.0 \u2192 281.0 for bromotetrandrine and IS, respectively. The present method exhibited good linearity over the concentration range of 20\u20135000\u00a0ng\/mL for bromotetrandrine in rat plasma with a lower limit of quantification of 20\u00a0ng\/mL. The intra\u2010 and inter\u2010day precisions were 2.8\u20137.5% and 3.2\u20138.1%, and the intra\u2010 and inter\u2010day accuracy ranged from \u22124.8 to 8.2% and \u22125.6 to 6.2%, respectively. The method was successfully applied to a pharmacokinetic study after a single oral administration to SD rats with bromotetrandrine of 50\u00a0mg\/kg. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1163","type":"journal-article","created":{"date-parts":[[2009,3,10]],"date-time":"2009-03-10T02:45:10Z","timestamp":1236653110000},"page":"623-629","source":"Crossref","is-referenced-by-count":4,"title":["Liquid chromatographic\/mass spectrometry assay of bromotetrandrine in rat plasma and its application to pharmacokinetic study"],"prefix":"10.1002","volume":"23","author":[{"given":"Naining","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quansheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changxiao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,3,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-004-0868-0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2574499"},{"key":"e_1_2_1_4_1","first-page":"301","article-title":"Pharmacokinetics of bromotetrandrine in rats and Beagle dogs","volume":"39","author":"Xiao SH","year":"2004","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_1_5_1","first-page":"63","article-title":"Studies on pharmacokinetics of bromotetrandrine in animals (II) Effect of coadministration of bromotetrandrine with adriamycin on the pharmacokinetics","volume":"4","author":"Xiao SH","year":"2004","journal-title":"Asian Journal of Drug Metabolism and Pharmacokinetics"},{"key":"e_1_2_1_6_1","first-page":"1221","article-title":"Studies on toxicokinetics of single or combination administration of bromotetrandrine and adriamycin in beagle dogs","volume":"9","author":"Xiao SH","year":"2004","journal-title":"Chinese Journal of Clinical Pharmacology and Therapeutics"},{"key":"e_1_2_1_7_1","first-page":"453","article-title":"Tissue distribution and excretion of bromotetrandrine in rats","volume":"40","author":"Xiao SH","year":"2005","journal-title":"Acta Pharmaceutica Sinica"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1163","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:29:25Z","timestamp":1760038165000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3,11]]},"references-count":6,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1002\/bmc.1163"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1163","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2009,3,11]]}}}