{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:39:05Z","timestamp":1760888345759,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,4,29]],"date-time":"2009-04-29T00:00:00Z","timestamp":1240963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Elevated levels of<jats:italic> trans<\/jats:italic>\u2010indolyl\u20103\u2010acryloylglycine (IAcrGly) have been reported in the urine of people with various conditions including pervasive developmental disorders (PDDs) such as autism and Asperger syndrome. Reversed\u2010phase high\u2010performance liquid chromatography with ultra\u2010violet detection using traditional particle silica\u2010based columns subsequent to solid\u2010phase extraction (SPE) has been the preferred assay method; requiring long analytical run times, high flow rates and high solvent usage. Recent developments in monolithic HPLC column technology facilitated the development of a novel analytical method, for the detection and quantification of urinary IAcrGly. The revised method eliminates the requirement for SPE pre\u2010treatment, reduces sample run\u2010time and decreases solvent volumes. Five urine samples from people diagnosed with PDD were run in quadruplicate to test the intra\u2010 and inter\u2010day reliability of the new method based on retention time, peak area and peak height for IAcrGly. Detection was by UV with IAcrGly confirmation by MS\/MS\u2010MS. Relative standard deviations showed significant improvement with the new method for all parameters. The new method represents a major advancement in the detection and quantification of IAcrGly by reducing time and cost of analysis whilst improving detection limits and reproducibility. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1231","type":"journal-article","created":{"date-parts":[[2009,4,29]],"date-time":"2009-04-29T03:41:53Z","timestamp":1240976513000},"page":"1108-1115","source":"Crossref","is-referenced-by-count":8,"title":["Development and reproducibility of a novel high\u2010performance liquid\u2010chromatography monolithic column method for the detection and quantification of <i>trans<\/i>\u2010indolyl\u20103\u2010acryloylglycine in human urine"],"prefix":"10.1002","volume":"23","author":[{"given":"Kevin","family":"Carr","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Whiteley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Shattock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2788.2003.00554.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1211\/0022357021778349"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01837709"},{"key":"e_1_2_1_5_1","first-page":"CR2","article-title":"Indolyl\u20103\u2010acryloylglycine (IAG) is a putative diagnostic urinary marker for autism spectrum disorders","volume":"9","author":"Bull G","year":"2003","journal-title":"Medical Science Monitoring"},{"key":"e_1_2_1_6_1","first-page":"1283","volume-title":"The Metabolic Basis of Inherited Disease","author":"Jepson JB.","year":"1965"},{"key":"e_1_2_1_7_1","first-page":"217","article-title":"Autistic disturbances of affective contact","volume":"2","author":"Kanner L.","year":"1943","journal-title":"Nervous Children"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","unstructured":"MarklovaE.Where does indolylacrylic acid come from?Amino Acids1999;17:401\u2013413.","DOI":"10.1007\/BF01361665"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(75)90354-X"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00153-4"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.09.045"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.6.2.175"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1362361302006003008"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1362361399003001005"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","unstructured":"WhiteleyPandShattockP.What makes trans\u2010indolyl\u20103\u2010acrloylglycine (IAcrGly) identified by high\u2010performance liquid\u2010chromatography (HPLC) relevant to pervasive developmental disorders?Journal of Nutrition and Environmental Medicine2003;13:1\u20137.","DOI":"10.1080\/13590840310001641996"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.2006.02207.x"},{"volume-title":"Tenth Revision of the International Classification of Diseases and Related Health Problems. 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