{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:22:26Z","timestamp":1790803346138,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,5,14]],"date-time":"2009-05-14T00:00:00Z","timestamp":1242259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A highly sensitive and selective on\u2010line two\u2010dimensional reversed\u2010phase liquid chromatography\/electrospray ionization\u2013tandem mass spectrometry (2D\u2010LC\u2010ESI\/MS\/MS) method was developed and validated to determine rifaximin in rat serum by direct injection. The 2D\u2010LC\u2010ESI\/MS\/MS system consisted of a restricted access media column for trapping proteins as the first dimension and a Waters C<jats:sub>18 <\/jats:sub>column as second dimension using 0.1% aqueous acetic acid:acetonitrile as mobile phase in a gradient elution mode. Rifampacin was used as an internal standard. The linear dynamic range was 0.5\u201310\u00a0ng\/mL (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0&gt;\u00a00.998). Acceptable precision and accuracy were obtained over the calibration range. The assay was successfully used in analysis of rat serum to support pharmacokinetic studies. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1236","type":"journal-article","created":{"date-parts":[[2009,5,14]],"date-time":"2009-05-14T02:50:53Z","timestamp":1242269453000},"page":"1145-1150","source":"Crossref","is-referenced-by-count":19,"title":["On\u2010line 2D\u2010LC\u2010ESI\/MS\/MS determination of rifaximin in rat serum"],"prefix":"10.1002","volume":"23","author":[{"given":"R. Nageswara","family":"Rao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Mastan","family":"Vali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhananjay D.","family":"Shinde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,5,14]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"51","article-title":"Pharmacokinetic study of rifaximin after oral administration in healthy volunteers","volume":"14","author":"Descombe JJ","year":"1994","journal-title":"International Journal of Clinical Pharmacology Research"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400888"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1999.00553.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(93)90004-Q"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2004.05.019"},{"key":"e_1_2_1_7_1","unstructured":"ICH.Validation of Analytical Procedures: Methodology. ICH Guidelines Q2B. Federal Registrar 62 1997; no. 96 27463."},{"key":"e_1_2_1_8_1","first-page":"333","article-title":"Separation and determination of rifaximin and Quinone\u2010rifaximin by HPLC","volume":"24","author":"Lv J","year":"2004","journal-title":"Chinese Journal of Pharmaceutical Analysis"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr025556v"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","unstructured":"RaoRN ShindeDDandAgawaneSB.Rapid determination of rifaximin in rat serum and urine by direct injection on to a shielded hydrophobic stationary phase by HPLC.Biomedical Chromatography2009(in press). doi:10.1002\/bmc.1149.","DOI":"10.1002\/bmc.1149"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.curtheres.2004.10.002"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0011-393X(97)80027-6"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000238165"},{"key":"e_1_2_1_14_1","first-page":"257","article-title":"In vitro and in vivo evaluation of L\/105, a new topical intestinal rifamycin","volume":"5","author":"Venturini AP","year":"1986","journal-title":"Chemioterapia"},{"key":"e_1_2_1_15_1","first-page":"161","article-title":"Evaluation of rifaximin, placebo and lactulose in reducing the levels of benzodiazepine\u2010like compounds in patients with liver cirrhosis: a pilot study","volume":"31","author":"Venturini I","year":"2005","journal-title":"Drugs Under Experimental and Clinical Research"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac040022u"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac010627f"},{"key":"e_1_2_1_18_1","first-page":"932","article-title":"RP\u2010HPLC determination of rifaximin","volume":"12","author":"Wang J","year":"2003","journal-title":"Chinese Journal of New Drugs"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr049852e"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.12.001"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1236","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1236","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:56:13Z","timestamp":1760057773000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1236"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5,14]]},"references-count":19,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["10.1002\/bmc.1236"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1236","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5,14]]}}}