{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T14:56:52Z","timestamp":1760799412639,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,5,14]],"date-time":"2009-05-14T00:00:00Z","timestamp":1242259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Selenomethionine (SeMet) is a widely used nutritional supplement that has potential benefit for people living in selenium\u2010deficient areas. Previous research has shown that selenium administered as SeMet undergoes significant enterohepatic recycling which may involve the gut microflora. In order to investigate this we have developed a simple method for the quantitation of <jats:sc>l<\/jats:sc>\u2010SeMet in rat gut content suspensions prepared from jejunum, ileum, caecum and colon. After incubation of <jats:sc>l<\/jats:sc>\u2010SeMet with gut content suspensions, samples were deproteinized with sulfosalicylic acid and derivatized with <jats:italic>o<\/jats:italic>\u2010phthaldialdehyde (OPA) and <jats:italic>N\u2010<\/jats:italic>acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine (NAC). Mass spectrometry confirmed the formation of a 1:1:1 derivative of <jats:sc>l<\/jats:sc>\u2010SeMet with OPA and NAC. Samples were analysed by reversed\u2010phase high\u2010performance liquid chromatography with fluorescence detection. The assay was linear in the concentration range 0.5\u2013100\u00a0\u00b5g\/mL (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a00.9992) with a limit of detection of 0.025\u00a0\u00b5g\/mL (signal\u2010to\u2010noise ratio of 5). Intra\u2010day and inter\u2010day accuracies were 91.1\u201392.8 and 91.7\u201395.5%, respectively with corresponding precisions as relative standard deviation of &lt;5%. Incubation of <jats:sc>l<\/jats:sc>\u2010SeMet with gut content suspensions from different parts of the rat intestine showed that <jats:sc>l<\/jats:sc>\u2010SeMet metabolism occurs mainly in the caecum. 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