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After samples were extracted with 10% (v\/v) methanol in acetonitrile and cleaned by solid\u2010phase extraction, chromatographic separation was performed on an IonPac\u00ae AS11 analytical column (250\u00a0\u00d7\u00a04.0\u00a0mm) using gradient KOH eluent with 10% (v\/v) methanol as organic modifier. Confirmation was depended on the extensive fragmentation of the indandione molecule under MS\/MS conditions which provides sufficient structural information. Quantification was performed by negative electrospray ionization in multiple reaction monitoring mode. All the method parameters were validated. It was confirmed that this method could be used in clinical diagnosis and forensic toxicology. 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