{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T10:47:51Z","timestamp":1775126871526,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Twelve chiral derivatizing reagents (CDRs) were synthesized by substituting one of the fluorine atoms in 1,5\u2010difluoro\u20102,4\u2010dinitrobenzene (DFDNB) with three optically pure amines [(<jats:italic>R<\/jats:italic>)\u2010(\u2212)\u20101\u2010cyclohexylethylamine, (+)\u2010dehydroabietylamine and (<jats:italic>S<\/jats:italic>)\u2010(\u2212)\u2010<jats:italic>\u03b1<\/jats:italic>,4\u2010dimethylbenzylamine], six amino acid amides [<jats:sc>l<\/jats:sc>\u2010Ala\u2010NH<jats:sub>2<\/jats:sub>, <jats:sc>l<\/jats:sc>\u2010Phe\u2010NH<jats:sub>2<\/jats:sub>, <jats:sc>l<\/jats:sc>\u2010Val\u2010NH<jats:sub>2<\/jats:sub>, <jats:sc>l<\/jats:sc>\u2010Leu\u2010NH<jats:sub>2<\/jats:sub>, <jats:sc>l<\/jats:sc>\u2010Met\u2010NH<jats:sub>2<\/jats:sub> and <jats:sc>d\u2010<\/jats:sc>Phg\u2010NH<jats:sub>2<\/jats:sub>] and three amino acids [<jats:sc>l<\/jats:sc>\u2010Ala, <jats:sc>l<\/jats:sc>\u2010Val and <jats:sc>l<\/jats:sc>\u2010Leu]. In addition, dinitrophenyl\u2010<jats:sc>l<\/jats:sc>\u2010Pro\u2010<jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide ester and <jats:italic>N\u2010<\/jats:italic>succinimidyl\u2010(<jats:italic>S<\/jats:italic>)\u20102\u2010(6\u2010methoxynaphth\u20102\u2010yl) propionate were also synthesized and used as CDR. Keeping in view the presence of an amino group, diastereomers of six <jats:italic>\u03b2<\/jats:italic>\u2010blockers (atenolol, propranolol, bisoprolol, metoprolol, salbutamol, and carvedilol) were synthesized by reaction with these 14 CDRs. The diastereomers were separated by RP\u2010HPLC. The method was validated for linearity, accuracy, limit of detection and limit of quantification. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1252","type":"journal-article","created":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T02:02:31Z","timestamp":1243821751000},"page":"1291-1299","source":"Crossref","is-referenced-by-count":31,"title":["Reversed\u2010phase high\u2010performance liquid chromatographic enantioresolution of six <i> \u03b2<\/i>\u2010blockers using dinitrophenyl\u2010<scp>l<\/scp>\u2010Pro\u2010<i>N\u2010<\/i>hydroxysuccinimide ester, <i>N\u2010<\/i>succinimidyl\u2010(<i>S<\/i>)\u20102\u2010(6\u2010methoxynaphth\u20102\u2010yl) propionate and twelve variants of Sanger's reagent as chiral derivatizing 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