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Signal intensity was high using ESI<jats:sup>+<\/jats:sup> source provided for the quantitation of samples. Chromatographic separation was performed on phenomenax C\u201018 column (250 \u00d7 4.60\u2009mm 5microns).Mobile phase contains acetonitrile, water (80; 20 v\/v) + 0.1% acetic acid, flow rate 1\u2009mL\/min.The retention time of Sirolimus 8.4\u2009min, the total run time10\u2009min. Linearity correlation coefficients (r<jats:sup>2<\/jats:sup>) curve was 0.997183.calibraction range 10\u20131000\u2009ng\/mL. The UV detection of Sirolimus was at 278(277.78) nm. Sirolimus coated drug eluting stents, MRM (Multiple reaction monitoring) transition of Sirolimus <jats:italic>m\/z<\/jats:italic> 936.83\u2013208.84 was selected to obtain maximum sensitivity. LC\/MS\/MS results exhibited consistency in drug content on the stent surface. <jats:italic>In\u2010vitro<\/jats:italic> release kinetic indicated the release of Sirolimus in 41 days from the date of implanted. Drug release was found at the first day, burst release was observed at 7<jats:sup>th<\/jats:sup> day of implantation. This study involved pharmacological coating of stents, based on the notion that sustained systemic local delivery of anti\u2010proliferative agents. LC\u2010MS\/MS method has been successfully used in the pharmacokinetic analysis of Sirolimus coated drug eluting stents. 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