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The influences of several factors (surfactant and buffer concentration, pH, applied voltage, capillary temperature and injection time) were studied. Using tyramine hydrochloride as internal standard, the analytes were all separated in about 4\u2009min. The separation was carried out in reversed polarity mode at 28\u00b0C, 25\u2009kV and using hydrodynamic injection (15\u2009s). The separation was effected in a fused\u2010silica capillary 100\u2009\u03bcm \u00d7 56\u2009cm and a background electrolyte of 20\u2009mM citric acid\u20131\u2009<jats:sc>M<\/jats:sc> Tris solution (pH 2.75), containing 125\u2009m<jats:sc>M<\/jats:sc> sodium dodecyl sulphate and detection at 254\u2009nm. The method was validated with respect to linearity, limit of detection and quantification, accuracy, precision and selectivity. Calibration curves were linear over the range 0.1\u20131\u2009\u03bcg\/mL (guanine) and from 0.1 to 120\u2009\u03bcg\/mL for both valacyclovir and acyclovir. The relative standard deviations of intra\u2010 and inter\u2010day migration times and corrected peak areas were less than 5.0%. The proposed method was successfully applied to the determination of the analytes in tablets and creams. From the previous study it is concluded that the stability\u2010indicating method developed for acyclovir and valacyclovir can be used for analysis of the drug in various stability samples. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1323","type":"journal-article","created":{"date-parts":[[2009,9,8]],"date-time":"2009-09-08T23:59:11Z","timestamp":1252454351000},"page":"535-543","source":"Crossref","is-referenced-by-count":25,"title":["Assay and stability\u2010indicating micellar electrokinetic chromatography method for the simultaneous determination of valacyclovir, acyclovir and their major impurity guanine in pharmaceutical formulations"],"prefix":"10.1002","volume":"24","author":[{"given":"Khaldun M.","family":"Al Azzam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahruddin","family":"Saad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Makahleah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan Y.","family":"Aboul\u2010Enein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdalla A.","family":"Elbashir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,9,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.11.051"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2006.02.031"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.11.038"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00488-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-827X(02)01237-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-827X(03)00157-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-3542(95)00066-6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00037-6"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00120-4"},{"key":"e_1_2_1_11_1","volume-title":"Capillary Electrophoresis in Chiral Analysis","author":"Chankvetadze B","year":"1997"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.farmac.2005.04.003"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.09.029"},{"key":"e_1_2_1_14_1","unstructured":"European Pharmacopoeia Vol. 2 5th edn 2005; 921."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00252-6"},{"key":"e_1_2_1_16_1","unstructured":"FieldenKE. 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