{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T15:41:06Z","timestamp":1774366866475,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,9,30]],"date-time":"2009-09-30T00:00:00Z","timestamp":1254268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this work, a simple, reproducible and sensitive micellar electrokinetic chromatography method was developed for the separation and determination of three coumarins, imperatorin (IM), isoimperatorin (IO) and osthole (OS) from traditional Chinese medicine and human serum. Field\u2010enhanced sample injection with reverse migrating micelles was used for on\u2010line concentration of the coumarins. The optimum buffer contained 50\u2009mM H<jats:sub>3<\/jats:sub>PO<jats:sub>4<\/jats:sub>, 160\u2009mM sodium dodecyl sulfate, 20% acetonitrile and 15% 2\u2010propanol, and the pH of buffer was 2.0. The sample solution was diluted with water containing 5\u2009mM sodium dodecyl sulfate and injected for 15\u2009s with \u22128\u2009kV after injection of 2\u2009s water plug. The effects of concentrations of sodium dodecyl sulfate and organic modifier, the sample matrix, the injection time of water plug, the injection voltage and injection time of sample on the separation and stacking efficiency were investigated. Under the optimum conditions, the analytes were well separated and by optimizing the stacking conditions, about 93, 195 and 136 fold improvement in the detection sensitivity was obtained for IM, IO and OS. The contents of three coumarins in <jats:italic>Angelica dahurica<\/jats:italic> Benth, Radix Angelicae Pubescentis and Fructus Cnidii were successfully determined with satisfactory repeatability and recovery. The possibilities of using this method for the determination of three coumarins in spiked human serum were also tested. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1330","type":"journal-article","created":{"date-parts":[[2009,9,30]],"date-time":"2009-09-30T03:53:17Z","timestamp":1254282797000},"page":"581-587","source":"Crossref","is-referenced-by-count":16,"title":["On\u2010line concentration by field\u2010enhanced sample injection with reverse migrating micelles in micellar electrokinetic capillary chromatography for the analysis of coumarins from traditional Chinese medicine and human serum"],"prefix":"10.1002","volume":"24","author":[{"given":"Ting\u2010Fu","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Hua","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan\u2010Hong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei\u2010E","family":"Yue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,9,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-41112"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00024a012"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00032a721"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac950650z"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(03)00184-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1055\/s-2000-14894","article-title":"Anti\u2010inflammol\/latory activity of coumarins from Decatropis bicolor on TPA ear mice model","volume":"66","author":"Garc\u00eda\u2010Arg\u00e1ez AN","year":"2000","journal-title":"Planta Medica"},{"key":"e_1_2_1_8_1","first-page":"159","article-title":"Cytotoxic compounds from Zanthoxylum americanum","volume":"5","author":"Ju Y","year":"2000","journal-title":"Tsinghua Science and Technology"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200210)23:19<3392::AID-ELPS3392>3.0.CO;2-X"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2000-9908"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00498-6"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00796-6"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9706281"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9711504"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.06.015"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-37716"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(96)00332-9"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1565(199703)8:2<74::AID-PCA332>3.0.CO;2-8"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-39697"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.08.032"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.01.023"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.09.070"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2000-8558"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.451"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2007.12.009"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1565(199811\/12)9:6<283::AID-PCA419>3.0.CO;2-9"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1330","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1330","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:29:14Z","timestamp":1760038154000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,30]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["10.1002\/bmc.1330"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1330","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,30]]}}}