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The analyte and the IS were extracted from plasma by LLE (diethyl ether\u2013dichloromethane, 80:20, v\/v) and analyzed by HPLC\u2010ESI\u2010MS\/MS. Chromatographic separation was performed using a gradient of methanol from 35 to 90% with 2.5\u2009m<jats:sc>m<\/jats:sc> NH<jats:sub>4<\/jats:sub>OH on a Gemini Phenomenex C<jats:sub>8<\/jats:sub> 5\u2009\u03bcm column (50 \u00d7 4.6\u2009mm i.d.) in 5.0\u2009min\/run. The method fitted to a linear calibration curve (0.05\u201310\u2009ng\/mL, <jats:italic>R<\/jats:italic> &gt; 0.9991). The precision (%CV) and accuracy ranged, respectively: intra\u2010batch from 1.5 to 6.8% and 99.1 to 106.6%, and inter\u2010batch from 2.4 to 9.0%, and 99.9 to 103.1%. The validated bioanalytical procedure was used to assess the comparative bioavailability in healthy volunteers of two dexchlorpheniramine 2.0\u2009mg tablet formulations (test dexchlorpheniramine, Eurofarma, and reference Celestamine<jats:sup>\u00ae<\/jats:sup>, Schering\u2010Plough). The study was conducted using an open, randomized, two\u2010period crossover design with a 2 week washout interval. Since the 90% confidence interval for <jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub> and AUC ratios were all within the 80\u2013125% interval proposed by ANVISA and FDA, it was concluded that test and reference formulations are bioequivalent concerning the rate and the extent of absorption. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1362","type":"journal-article","created":{"date-parts":[[2009,11,18]],"date-time":"2009-11-18T06:02:08Z","timestamp":1258524128000},"page":"774-781","source":"Crossref","is-referenced-by-count":15,"title":["Determination of chlorpheniramine in human plasma by HPLC\u2010ESI\u2010MS\/MS: application to a dexchlorpheniramine comparative bioavailability study"],"prefix":"10.1002","volume":"24","author":[{"given":"Ronilson Agnaldo","family":"Moreno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diogo","family":"Oliveira\u2010Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Eduardo","family":"Sverdloff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Carter","family":"Borges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Alexandre","family":"Rebelo Galvinas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael Barrientos","family":"Astigarraga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ney Carter","family":"Borges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,11,18]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"ANVISA Brazilian National Sanitary Surveillance Agency Brazil.2003."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)81522-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19990630)13:12<1175::AID-RCM639>3.0.CO;2-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00043764-200101000-00013"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)01252-2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.311"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(01)00570-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0274(199608)30:2<234::AID-AJIM16>3.0.CO;2-Y"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00616-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1185\/0300799019117014"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920006777697981"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(00)00163-X"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/js980045m"},{"key":"e_1_2_1_15_1","first-page":"122","article-title":"Tandem mass spectrometry (LC\u2010MS\u2010MS): a predominant role in bioassays for pharmacokinetic studies","volume":"57","author":"Marzo A","year":"2007","journal-title":"Arzneimittel\u2010Forschung\u2014Drug Research"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)83598-7"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac020361s"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-007-1248-5"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80155-X"},{"key":"e_1_2_1_20_1","first-page":"14","article-title":"Effects of terfenadine, diphenhydramine, and placebo on skills performance","volume":"42","author":"Moskowitz H","year":"1988","journal-title":"Cutis"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00142-3"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-2918(97)80071-9"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1995.tb01140.x"},{"key":"e_1_2_1_24_1","first-page":"666","article-title":"The clinical safety of H1\u2010receptor antagonists. 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