{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T21:00:46Z","timestamp":1778792446566,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,11,10]],"date-time":"2009-11-10T00:00:00Z","timestamp":1257811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A liquid chromatography\u2013photodiode array (LC\u2010PDA) method using a chiral analytical column was developed to determine the plasma levels of enantiomers of methadone and its chiral metabolite, 2\u2010ethylidene\u20101,5\u2010dimethyl\u20103,3\u2010diphenylpyrrolidine (EDDP), without the standard compounds of <jats:italic>R<\/jats:italic>\u2010form or <jats:italic>S<\/jats:italic>\u2010form enantiomers. This method was established by the characteristics of recombinant cytochrome P\u2010450 (CYP) isozymes, where CYP2C19 prefers to metabolize <jats:italic>R<\/jats:italic>\u2010methadone and CYP2B6 prefers to metabolize <jats:italic>S<\/jats:italic>\u2010methadone. We incubated the racemic methadone standard with either enzyme for 24\u2009h. We identified the retention times of <jats:italic>R<\/jats:italic>\u2010 and <jats:italic>S<\/jats:italic>\u2010methadone to be around 10.72 and 14.46\u2009min, respectively. Furthermore, we determined the retention times of <jats:italic>R<\/jats:italic>\u2010 and <jats:italic>S<\/jats:italic>\u2010EDDP to be approximately 6.76 and 7.72\u2009min, respectively. No interferences were shown through the retention times of morphine, buprenorphine and diazepam. With the high recovery rate of a solid\u2010phase extraction procedure, this method was applied in analyzing plasma concentrations of seven methadone maintenance patients where <jats:italic>R<\/jats:italic>\u2010 and <jats:italic>S<\/jats:italic>\u2010methadone and <jats:italic>R<\/jats:italic>\u2010 and <jats:italic>S<\/jats:italic>\u2010EDDP were 233.4 \u00b1 154.9 and 185.9 \u00b1 136.3\u2009ng\/mL and 84.4 \u00b1 99.4 and 37.6 \u00b1 22.9\u2009ng\/mL, respectively. These data suggest that the present method can be applied for routine assay for plasma methadone and EDDP concentrations for patients under treatment. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1363","type":"journal-article","created":{"date-parts":[[2009,11,10]],"date-time":"2009-11-10T10:49:43Z","timestamp":1257850183000},"page":"782-788","source":"Crossref","is-referenced-by-count":25,"title":["Development of a method to measure methadone enantiomers and its metabolites without enantiomer standard compounds for the plasma of methadone maintenance patients"],"prefix":"10.1002","volume":"24","author":[{"given":"Sheng\u2010Chang","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ing\u2010Kang","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiow\u2010Ling","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu Chih","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiang\u2010Wei","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keh\u2010Ming","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Li","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,11,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00551-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1592\/phco.28.7.863"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clpt.2005.08.011"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200241140-00003"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2005.09.010"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1216"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e31811f1bbe"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.10303"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.10.048"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.10.053"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-199802000-00007"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-199606000-00001"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2005.09.009"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1369-1600.2006.00014.x"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.08.153"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-636X(1999)11:4<319::AID-CHIR10>3.0.CO;2-Y"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.11.4148-4153.2004"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e3181642938"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200301621"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.31.6.742"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-003-1718-1"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1363","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1363","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:27:59Z","timestamp":1760038079000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,10]]},"references-count":21,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2010,7]]}},"alternative-id":["10.1002\/bmc.1363"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1363","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,11,10]]}}}