{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:08:41Z","timestamp":1787166521988,"version":"build-2736575974"},"reference-count":14,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,10,13]],"date-time":"2010-10-13T00:00:00Z","timestamp":1286928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2011,7]]},"abstract":"Abstract<\/jats:title>\n \n In this study, a new peptide named BmK AGP\u2010SYPU1 with an analgesic effect was purified from the venom of Chinese scorpion\n Buthus martensi<\/jats:italic>\n Karsch (BmK) through a four\u2010step chromatographic process. The mouse twisting test was used to identify the target peptides in every separation step. The purified BmK AGP\u2010SYPU1 was further qualified by RP\u2010HPLC and HPCE. The molecular mass determined by the MALDI\u20104800\u2010TOF\/TOF MS for BmK AGP\u2010SYPU1 was 7544\u2009Da. Its primary structure of the N\u2010terminal was obtained using Edman degradation. The gene sequence of BmK AGP\u2010SYPU1 was cloned from the cDNA pool and genomic of scorpion glands, respectively, and then expressed in\n Escherichia coli<\/jats:italic>\n . The sequence determination showed that BmK AGP\u2010SYPU1 was composed of 66 amino acid residues with a new primary structure. The metal chelating affinity column and cation exchange chromatography were used to purify the recombinant BmK AGP\u2010SYPU1. Consequently, the native and recombinant BmK AGP\u2010SYPU1 showed similar analgesic effects on mice as assayed using a mouse twisting model. These results suggested that BmK AGP\u2010SYPU1 is a new analgesic component found in the Chinese scorpion\n Buthus martensi<\/jats:italic>\n Karsch. Copyright \u00a9 2010 John Wiley & Sons, Ltd.\n <\/jats:p>","DOI":"10.1002\/bmc.1519","type":"journal-article","created":{"date-parts":[[2010,10,14]],"date-time":"2010-10-14T09:45:58Z","timestamp":1287049558000},"page":"801-807","source":"Crossref","is-referenced-by-count":27,"title":["Purification, characterization and functional expression of a new peptide with an analgesic effect from Chinese scorpion\n Buthus martensii<\/i>\n Karsch (BmK AGP\u2010SYPU1)"],"prefix":"10.1002","volume":"25","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Bo","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Feng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Fu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010Hai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,10,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti770"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90559-L"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.2004.00164.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2010.03.017"},{"key":"e_1_2_6_6_1","first-page":"76","volume-title":"Methods in Narcotics Research","author":"Fennessy MR","year":"1975"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444900006004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(01)00241-2"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3011.2001.00869.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00221-9"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.00395.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s008940100045"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-5928(02)00609-5"},{"key":"e_1_2_6_14_1","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(00)00355-5"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1519","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1519","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:46:46Z","timestamp":1760057206000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1519"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,13]]},"references-count":14,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1002\/bmc.1519"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1519","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,13]]}}}