{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:41:43Z","timestamp":1760888503219,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,11,5]],"date-time":"2010-11-05T00:00:00Z","timestamp":1288915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple and sensitive high\u2010performance liquid chromatographic method was developed and validated for simultaneous estimation of pioglitazone and felodipine in pig serum. The present method consists of protein precipitation, extraction of analytes from pig serum into dichloromethane and separation using reversed\u2010phase C<jats:sub>18<\/jats:sub> column. Nitrendipine was used as an internal standard and the eluent was monitored by UV detector at 240\u2009nm. The mobile phase used was acetonitrile and 50\u2009m<jats:sc>m<\/jats:sc> ammonium acetate buffer at a flow rate of 1\u2009mL\/min. The retention times for pioglitazone, felodipine and nitrendipine were found to be 5.12, 10.53 and 7.14\u2009min, respectively. The intraday and inter\u2010day coefficient of variation and percent error values of assay method were less than 7% and mean recovery was more than 94% for each analyte, and the method was found to be precise, accurate and specific during study. The method was successfully applied for pharmacokinetic study of pioglitazone and felodipine from bioadhesive buccal tablet after buccal administration to pigs. The <jats:italic>C<\/jats:italic><jats:sub>Max<\/jats:sub>, <jats:italic>T<\/jats:italic><jats:sub>Max<\/jats:sub>, and AUC<jats:sub>0\u201324<\/jats:sub> of pioglitazone and felodipine from buccal tablet were found to be 394.6\u2009ng\/mL, 5.6\u2009h, 2624.2\u2009ng\u2009h\/mL and 44.4\u2009ng\/mL, 5.5\u2009h, 275.8\u2009ng\u2009h\/mL, respectively. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1553","type":"journal-article","created":{"date-parts":[[2010,11,7]],"date-time":"2010-11-07T08:43:44Z","timestamp":1289119424000},"page":"952-958","source":"Crossref","is-referenced-by-count":7,"title":["Development of a high\u2010performance liquid chromatography method for simultaneous determination of pioglitazone and felodipine in pig serum: application to pharmacokinetic study"],"prefix":"10.1002","volume":"25","author":[{"given":"Chinna Reddy","family":"Palem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh","family":"Gannu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shravan Kumar","family":"Yamsani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vamshi Vishnu","family":"Yamsani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhusudan Rao","family":"Yamsani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,11,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(84)80055-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)83809-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(94)00579-T"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2000-8525"},{"key":"e_1_2_6_6_1","first-page":"388","article-title":"Pharmacokinetics and haemodynamic effects of felodipine as monotherapy in hypertensive patients","volume":"10","author":"Edgar B","year":"1987","journal-title":"Clinical Investigative Medicine"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2008.10.011"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(92)80128-D"},{"volume-title":"Guidance for Industry: Bioanalytical Method Validation","year":"2001","author":"FDA","key":"e_1_2_6_9_1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(01)00600-6"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/03639040500215958"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345890680091101"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(01)00582-9"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)01070-5"},{"key":"e_1_2_6_15_1","first-page":"118","article-title":"Determination of pioglitazone hydrochloride in tablets by high\u2010performance liquid chromatography","volume":"9","author":"Lotfy Saber AMR","year":"2008","journal-title":"Pakistan Journal of Analytical and Environmental Chemistry"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.10.032"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.04.029"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(91)80386-Q"},{"first-page":"3088","volume-title":"Physician's Desk Reference","year":"2000","key":"e_1_2_6_19_1"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(02)00036-5"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)82393-2"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/156720107779314785"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00575-0"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1553","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1553","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:46:46Z","timestamp":1760057206000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1553"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,5]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2011,8]]}},"alternative-id":["10.1002\/bmc.1553"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1553","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2010,11,5]]}}}