{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:09:08Z","timestamp":1760141348643,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,12,10]],"date-time":"2010-12-10T00:00:00Z","timestamp":1291939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Coenzyme Q<jats:sub>10<\/jats:sub> (CoQ<jats:sub>10<\/jats:sub>) is a naturally occurring compound located in all membranes throughout the cell. A rapid and sensitive HPLC method was developed to determine the concentration of CoQ<jats:sub>10<\/jats:sub> in dog plasma using a surrogate matrix. Chromatographic separation was carried out on a Diamonsil C<jats:sub>18<\/jats:sub> column with the UV detector set at 275\u2009nm. Methanol\u20132\u2010propanol (40:60, v\/v) was used as a mobile phase delivered at a flow rate of 1.0\u2009mL\/min. Calibrators were prepared using blank plasma\u2013K<jats:sub>2<\/jats:sub>HPO<jats:sub>4<\/jats:sub> buffer (50\u2009m<jats:sc>m<\/jats:sc>, pH 8.0)\u2013saline (1:3:6, v\/v\/v) as surrogate matrix. It was shown that the surrogate matrix had similar properties to dog plasma for CoQ<jats:sub>10<\/jats:sub> in extraction, freeze\u2013thaw and stability. The assay was linear over the concentration range of 0.10\u2013100\u2009\u00b5g\/mL. The intra\u2010 and inter\u2010day precisions were within 13.3% in terms of relative standard deviation (RSD%) and the accuracy was within \u00b17.5% in terms of relative error. This simple and reproducible HPLC method with less plasma volume (0.4\u2009mL) and adequate sensitivity was successfully applied to pharmacokinetic studies of CoQ<jats:sub>10<\/jats:sub> in dogs and an investigation of the effect of CoQ<jats:sub>10<\/jats:sub> formulation on CoQ<jats:sub>10<\/jats:sub> baseline levels. 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