{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:42:06Z","timestamp":1760888526769,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,2,10]],"date-time":"2011-02-10T00:00:00Z","timestamp":1297296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A liquid chromatographic mass spectrometric (LC\u2010MS) assay for the quantification of nicotine and cotinine in human specimens was developed. Human serum and urine (100\u2009\u03bcL) were subjected to liquid\u2013liquid extraction. For glucuronidated cotinine, serum was alkalinized and hydrolyzed before extraction. The dried samples were reconstituted and run using gradient flow reverse\u2010phase liquid chromatography with MS detection. The ions utilized for quantification of nicotine, cotinine and milrinone (internal standard) were 162.8, 176.9 and 211.9\u2009<jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic>, respectively. The mean recoveries were over 80% for cotinine and nicotine with excellent linearity between nominal concentrations and peak area ratios, over a wide concentration range. The percentage coefficient of variation and mean error of the inter\u2010 and intra\u2010day validations were &lt;15% for nicotine and cotinine. Analysis of serum from cardiac patients receiving amiodarone suggested that a number of patients were either active smokers or exposed to second\u2010hand smoke. Significant concentrations of nicotine and cotinine were measured in the urine of a known smoking volunteer. The method was highly specific, sensitive and applicable as a tool in detecting and monitoring the passive exposure to tobacco smoke using small specimen volumes (0.1\u2009mL). Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1581","type":"journal-article","created":{"date-parts":[[2011,2,16]],"date-time":"2011-02-16T11:12:00Z","timestamp":1297854720000},"page":"1124-1131","source":"Crossref","is-referenced-by-count":14,"title":["A liquid chromatography\u2010mass spectrometry method for nicotine and cotinine; utility in screening tobacco exposure in patients taking amiodarone"],"prefix":"10.1002","volume":"25","author":[{"given":"Raniah Q.","family":"Gabr","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy and Pharmaceutical Sciences University of Alberta  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marwa E.","family":"Elsherbiny","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy and Pharmaceutical Sciences University of Alberta  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishwa","family":"Somayaji","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy and Pharmaceutical Sciences University of Alberta  Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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