{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T21:00:33Z","timestamp":1782594033982,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,2,4]],"date-time":"2011-02-04T00:00:00Z","timestamp":1296777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A simple and sensitive liquid chromatography\u2013electrospray ionization\u2013tandem mass spectrometry (LC\u2010ESI\u2010MS\/MS) technique was developed and validated for the determination of sibutramine and its <jats:italic>N<\/jats:italic>\u2010desmethyl metabolites (M1 and M2) in human plasma. After extraction with methyl <jats:italic>t<\/jats:italic>\u2010butyl ether, chromatographic separation of analytes in human plasma was performed using a reverse\u2010phase Luna C<jats:sub>18<\/jats:sub> column with a mobile phase of acetonitrile\u201310\u2009m<jats:sc>m<\/jats:sc> ammonium formate buffer (50:50, v\/v) and quantified by ESI\u2010MS\/MS detection in positive ion mode. The flow rate of the mobile phase was 200\u2009\u03bcL\/min and the retention times of sibutramine, M1, M2 and internal standard (chlorpheniramine) were 1.5, 1.4, 1.3 and 0.9\u2009min, respectively. The calibration curves were linear over the range 0.05\u201320\u2009ng\/mL, for sibutramine, M1 and M2. The lower limit of quantification was 0.05\u2009ng\/mL using 500\u2009\u03bcL of human plasma. The mean accuracy and the precision in the intra\u2010 and inter\u2010day validation for sibutramine, M1 and M2 were acceptable. This LC\u2010MS\/MS method showed improved sensitivity and a short run time for the quantification of sibutramine and its two active metabolites in plasma. The validated method was successfully applied to a pharmacokinetic study in human. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1587","type":"journal-article","created":{"date-parts":[[2011,10,6]],"date-time":"2011-10-06T00:34:07Z","timestamp":1317861247000},"page":"1181-1188","source":"Crossref","is-referenced-by-count":18,"title":["Simultaneous determination of sibutramine and its active metabolites in human plasma by LC\u2010MS\/MS and its application to a pharmacokinetic study"],"prefix":"10.1002","volume":"25","author":[{"given":"Jung\u2010Woo","family":"Bae","sequence":"first","affiliation":[{"name":"School of Pharmacy Sungkyunkwan University  Suwon 440\u2010746 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Ik","family":"Choi","sequence":"additional","affiliation":[{"name":"School of Pharmacy Sungkyunkwan University  Suwon 440\u2010746 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choon\u2010Gon","family":"Jang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Sungkyunkwan University  Suwon 440\u2010746 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok\u2010Yong","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Pharmacy Sungkyunkwan University  Suwon 440\u2010746 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,2,4]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"345","article-title":"A pilot study to evaluate the pharmacokinetics of sibutramine in healthy subjects under fasting and fed conditions","volume":"7","author":"Abolfathi Z","year":"2004","journal-title":"Journal of Pharmacy and Pharmacological Science"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.108.020727"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/45.2.91"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00818-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0091270010362906"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702446"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(03)00209-5"},{"key":"e_1_2_6_9_1","unstructured":"FDA.2009Sibutramine label information. 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