{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T02:09:24Z","timestamp":1771466964248,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,6,28]],"date-time":"2011-06-28T00:00:00Z","timestamp":1309219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>There is an increasing interest in potential medical applications of ayahuasca, a South American psychotropic plant tea with a long cultural history of indigenous medical and religious use. Clinical research into ayahuasca will require specific, sensitive and comprehensive methods for the characterization and quantitation of these compounds and their metabolites in blood. A combination of two analytical techniques (high\u2010performance liquid chromatography with ultraviolet and\/or fluorescence detection and gas chromatography with nitrogen\u2013phosphorus detection) has been used for the analysis of some of the constituents of ayahuasca in blood following its oral consumption. We report here a single methodology for the direct analysis of 14 of the major alkaloid components of ayahuasca, including several known and potential metabolites of <jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>\u2010dimethyltryptamine and the harmala alkaloids in blood. The method uses 96\u2010well plate\/protein precipitation\/filtration for plasma samples, and analysis by HPLC\u2013ion trap\u2013ion trap\u2013mass spectrometry using heated electrospray ionization to reduce matrix effects. The method expands the list of compounds capable of being monitored in blood following ayahuasca administration while providing a simplified approach to their analysis. The method has adequate sensitivity, specificity and reproducibility to make it useful for clinical research with ayahuasca. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1657","type":"journal-article","created":{"date-parts":[[2011,6,30]],"date-time":"2011-06-30T08:54:12Z","timestamp":1309424052000},"page":"301-313","source":"Crossref","is-referenced-by-count":43,"title":["Methodology for determining major constituents of ayahuasca and their metabolites in blood"],"prefix":"10.1002","volume":"26","author":[{"given":"Ethan H.","family":"McIlhenny","sequence":"first","affiliation":[{"name":"Department of Comparative Biomedical Sciences School of Veterinary Medicine Louisiana State University  Baton Rouge LA 70806 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Riba","sequence":"additional","affiliation":[{"name":"Centre d'Investigaci\u00f3 de Medicaments, Institut de Recerca, Servei de Farmacologia Cl\u00ednica, Hospital de Sant Pau, Barcelona, Departament de Farmacologia i Terap\u00e8utica Universitat Aut\u00f2noma de Barcelona, Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Salud Mental, CIBERSAM  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manel J.","family":"Barbanoj","sequence":"additional","affiliation":[{"name":"Centre d'Investigaci\u00f3 de Medicaments, Institut de Recerca, Servei de Farmacologia Cl\u00ednica, Hospital de Sant Pau, Barcelona, Departament de Farmacologia i Terap\u00e8utica Universitat Aut\u00f2noma de Barcelona, Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Salud Mental, CIBERSAM  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rick","family":"Strassman","sequence":"additional","affiliation":[{"name":"Department of Psychiatry School of Medicine University of New Mexico  Albuquerque NM 87131 USA"},{"name":"Cottonwood Research Foundation  Taos NM 87571 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven A.","family":"Barker","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedical Sciences School of Veterinary Medicine Louisiana State University  Baton Rouge LA 70806 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,6,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/02791072.1989.10472145"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(80)90169-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(08)60291-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/02791072.2005.10399796"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02245347"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/20.6.492"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(98)00168-8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/02791072.2005.10399795"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.12.030"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2010.03.024"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mp.4000526"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3223(86)90010-7"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.9771"},{"key":"e_1_2_7_15_1","first-page":"257","volume-title":"Yearbook of Cross\u2010cultural Medicine and Psychotherapy","author":"Mabit J","year":"1996"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.ajp.159.4.599"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac020361s"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.11.009"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.10.088"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1551"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2004.03.002"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/02791072.1984.10472305"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(84)90003-5"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gem016"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.1110"},{"key":"e_1_2_7_26_1","unstructured":"RibaJ.Human pharmacology of ayahuasca. 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