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A simple, rapid and reliable method was developed and validated to determine LDC and its aromatized derivative (LDCA) simultaneously in plasma. Puerarin was used as an internal standard (IS), and plasma samples were pretreated with one\u2010step precipitation by acetonitrile. Separation was achieved on a reverse\u2010phase C<jats:sub>18<\/jats:sub> column with a mobile phase composed of methanol and water containing 5\u2009m<jats:sc>m<\/jats:sc> ammonium acetate at pH 3.5 in gradient elution mode. Detection was performed on a triple quadrupole tandem mass spectrometer using electrospray ionization (ESI) by multiple reaction monitoring (MRM) in the negative ion mode. Good linearity was obtained over the concentration range of 0.2\u2013100\u2009\u00b5g\/mL for LDM. Precision and accuracy were validated by RSD% values in the range of 2.6\u201313.0% and RE% values between \u22124.6 and 3.8%, respectively. In addition, no specificity and matrix effects were observed. The recovery was found to be 99.2\u2013111.0% and stability in various conditions was found to be acceptable. This method was applied in preclinical pharmacokinetic studies for routine monitoring of LDM in rat plasma. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1676","type":"journal-article","created":{"date-parts":[[2011,8,9]],"date-time":"2011-08-09T08:52:30Z","timestamp":1312879950000},"page":"400-406","source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous determination of lidamycin enediyne chromophore (LDC) and its aromatized derivative (LDCA) using puerarin as internal standard in rat plasma by LC\u2010MS\/MS"],"prefix":"10.1002","volume":"26","author":[{"given":"Yanqing","family":"Wen","sequence":"first","affiliation":[{"name":"Laboratory of Drug Metabolism and Pharmacokinetics Beijing Institute of Blood Transfusion Medical Science  27 Taiping Road Beijing 100850 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuzhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100050 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