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The current study describes a method to analyze rabbit lipoproteins in plasma by anion\u2010exchange high\u2010performance liquid chromatography using a column filled with nonporous, diethylaminoethyl\u2010ligated polymers. A solution of NaClO<jats:sub>4<\/jats:sub> was used to adjust the ionic strength of the eluent. The method required only 15 \u03bcL of plasma and analysis was completed in 23\u2009min. Five lipoprotein fractions (high\u2010density lipoprotein, low\u2010density lipoprotein, intermediate\u2010density lipoprotein, very\u2010low\u2010density lipoprotein and chylomicrons) were eluted with step\u2010wise increases in a concentration of NaClO<jats:sub>4<\/jats:sub>. The post\u2010column eluate was reacted with an enzymatic reagent to determine total cholesterol, and the lipoprotein\u2010cholesterol fraction was calculated according to relative peak areas in the chromatogram. The within\u2010day and between\u2010day assay coefficients of variation for lipoprotein cholesterol levels ranged between 0.436 and 7.143% and between 2.905 and 10.526%, respectively. Administering a high\u2010fat diet increased lipoprotein\u2010cholesterol concentrations by 6\u2010 to 77\u2010fold. The method described here was nevertheless able to quantitate levels of lipoprotein\u2010cholesterol in plasma samples from these rabbits. These results indicate that this method may be applied to lipoprotein studies using hyperlipidemic rabbit models. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1683","type":"journal-article","created":{"date-parts":[[2011,8,12]],"date-time":"2011-08-12T09:31:00Z","timestamp":1313141460000},"page":"434-440","source":"Crossref","is-referenced-by-count":5,"title":["Anion\u2010exchange HPLC separation of five major rabbit lipoproteins using a nonporous diethylaminoethyl\u2010ligated gel with a perchlorate\u2010containing eluent"],"prefix":"10.1002","volume":"26","author":[{"given":"Yuji","family":"Hirowatari","sequence":"first","affiliation":[{"name":"Bioscience Division Tosoh Corporation  2743\u20101 Hayakawa Ayase\u2010shi Kanagawa 252\u20101123 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Kon","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine Juntendo University Faculty of Medicine  2\u20101\u20101 Hongo Bnkyo\u2010ku Tokyo 113\u20108421 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