{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:16:19Z","timestamp":1790813779623,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,10,27]],"date-time":"2011-10-27T00:00:00Z","timestamp":1319673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ultra high\u2010performance liquid chromatographic (UHPLC) systems on columns packed with materials ranging from 1.9 to 2.7\u2009\u00b5m average particle size were assessed for the fast and sensitive analysis of porphyrins in clinical materials. The fastest separation was achieved on an Agilent Poroshell C<jats:sub>18<\/jats:sub> column (2.7\u2009\u00b5m particle size, 50\u2009\u00d7\u20094.6\u2009mm i.d.), followed by a Thermo Hypersil Gold C<jats:sub>18<\/jats:sub> column (1.9\u2009\u00b5m particle size, 50\u2009\u00d7\u20092.1\u2009mm i.d.) and the Thermo Hypersil BDS C<jats:sub>18<\/jats:sub> column (2.4\u2009\u00b5m particle size, 100\u2009\u00d7\u20092.1\u2009mm i.d.). All columns required a mobile phase containing 1\u2009<jats:sc>m<\/jats:sc> ammonium acetate buffer, pH 5.16, with a mixture of acetonitrile and methanol as the organic modifiers for optimum resolution of the type I and III isomers, particularly for uroporphyrin I and III isomers. All UHPLC columns were suitable and superior to conventional HPLC columns packed with 5\u2009\u00b5m average particle size materials for clinical sample analysis. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1720","type":"journal-article","created":{"date-parts":[[2011,10,27]],"date-time":"2011-10-27T01:48:19Z","timestamp":1319680099000},"page":"714-719","source":"Crossref","is-referenced-by-count":17,"title":["Ultra high\u2010performance liquid chromatography of porphyrins in clinical materials: column and mobile phase selection and optimisation"],"prefix":"10.1002","volume":"26","author":[{"given":"Christopher M.","family":"Benton","sequence":"first","affiliation":[{"name":"Clinical Biochemistry King's College Hospital  Denmark Hill London SE5 9RS UK"},{"name":"Cancer Studies and Molecular Medicine, RKCSB University of Leicester  Leicester LE2 7LX UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang Kee","family":"Lim","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry King's College Hospital  Denmark Hill London SE5 9RS UK"},{"name":"MRC Bioanalytical Science Group, School of Biological Sciences and Chemical Sciences, Birkbeck University of London  Malet Street London WC1E 7HX UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caje","family":"Moniz","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry King's College Hospital  Denmark Hill London SE5 9RS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald JL","family":"Jones","sequence":"additional","affiliation":[{"name":"Cancer Studies and Molecular Medicine, RKCSB University of Leicester  Leicester LE2 7LX UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,10,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2009.08.023"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1662"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.656"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/46.3.254"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(03)00346-5"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1142\/9789814277198"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(84)90192-X"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)83869-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/31.7.1163"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408448509029323"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2005.06.035"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61925-5"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1872-0854(09)00006-X"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-2509(56)80003-1"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(89)90067-7"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.1720","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.1720","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:31:43Z","timestamp":1760034703000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.1720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,27]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1002\/bmc.1720"],"URL":"https:\/\/doi.org\/10.1002\/bmc.1720","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,27]]}}}