{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T10:47:35Z","timestamp":1774262855042,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,12,27]],"date-time":"2011-12-27T00:00:00Z","timestamp":1324944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Poria cocos<\/jats:italic> is a well\u2010known medicinal plant widely used in China and other East Asian countries owing to its various therapeutic effects. However, the bioactive constituents responsible for the pharmacological effects of <jats:italic>Poria cocos<\/jats:italic> and their metabolites <jats:italic>in vivo<\/jats:italic> are still unclear to date. The aim of the present study was to develop a practical method based on the combined use of the liquid chromatography coupled with electrospray ionization multistage tandem mass spectrometry (LC\u2010ESI\u2010MS<jats:italic><jats:sup>n<\/jats:sup><\/jats:italic>) for the comprehensive and systematic separation and characterization of the bioactive constituents of <jats:italic>Poria cocos<\/jats:italic> extract and their metabolites in rats. Based on the proposed strategy, a total of 34 compounds were characterized from the extract of <jats:italic>Poria cocos<\/jats:italic>. Among them, eight were unambiguously identified by comparing their retention times and mass spectra with those of reference standards, and 26 were tentatively identified on the basis of their MS<jats:italic><jats:sup>n<\/jats:sup><\/jats:italic> fragmentation behaviors and molecular weight information from literatures. <jats:italic>In vivo<\/jats:italic>, seven compounds were successfully detected in rat urine whereas one was found in rat plasma. This study proposed a series of potential bioactive components and provided helpful chemical information for further research on the action mechanism of traditional Chinese medicine. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.1756","type":"journal-article","created":{"date-parts":[[2011,12,27]],"date-time":"2011-12-27T23:19:14Z","timestamp":1325027954000},"page":"1109-1117","source":"Crossref","is-referenced-by-count":55,"title":["Systematic screening and characterization of the major bioactive components of <i>Poria cocos<\/i> and their metabolites in rats by LC\u2010ESI\u2010MS<i><sup>n<\/sup><\/i>"],"prefix":"10.1002","volume":"26","author":[{"given":"Yun","family":"Ling","sequence":"first","affiliation":[{"name":"Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingcang","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 People's Republic of 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