{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T13:52:52Z","timestamp":1782827572112,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,1,25]],"date-time":"2012-01-25T00:00:00Z","timestamp":1327449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Extracellular water (ECW) assessment is based on dilution techniques, commonly using blood sampling. However, plasma collection is an invasive procedure. We aimed to validate the use of saliva for ECW estimation by the bromide dilution technique using plasma as the reference method, in a sample of elite athletes. A total of 89 elite athletes with a mean age of 20.4\u2009\u00b1\u20094.4\u2009years were evaluated. Baseline samples were collected before sodium bromide oral dose administration, and enriched samples were collected 3\u2009h post\u2010dose administration. The bromide concentration was assessed by high\u2010performance liquid chromatography. Comparison of means, concordance coefficient correlation (CCC), multiple regression and Bland\u2013Altman analysis were performed. The ECW from saliva explained 91% of the variance in ECW by plasma with a standard error of estimation of 0.91\u2009kg. The CCC between alternative and reference methods was 0.952. No significant trend was observed between the mean and difference of the methods, with limits of agreement ranging between \u22121.5 and 2.1\u2009kg. These findings reveal that bromide dilution volume calculated from saliva samples is a valid noninvasive method for ECW assessment in elite athletes. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.2702","type":"journal-article","created":{"date-parts":[[2012,1,25]],"date-time":"2012-01-25T00:59:14Z","timestamp":1327453154000},"page":"1348-1352","source":"Crossref","is-referenced-by-count":11,"title":["Validity of extracellular water assessment with saliva samples using plasma as the reference biological fluid"],"prefix":"10.1002","volume":"26","author":[{"given":"Catarina N.","family":"Matias","sequence":"first","affiliation":[{"name":"Exercise and Health Laboratory Faculty of Human Kinetics\u2010Technical University of Lisbon  Estrada da Costa, 1499\u2010688 Cruz\u2010Quebrada Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Analiza M.","family":"Silva","sequence":"additional","affiliation":[{"name":"Exercise and Health Laboratory Faculty of Human Kinetics\u2010Technical University of Lisbon  Estrada da Costa, 1499\u2010688 Cruz\u2010Quebrada 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