{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T13:46:33Z","timestamp":1775483193619,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T00:00:00Z","timestamp":1331164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The disposition of the cannabimimetic naphthalen\u20101\u2010yl\u2010(1\u2010pentylindol\u20103\u2010yl)methanone (JWH\u2010018) in mice following inhalation of the smoke of the herbal incense product (HIP) \u2018Buzz\u2019 is presented. A high\u2010pressure liquid chromatography with electrospray ionization triple quadrupole mass spectrometer (HPLC\/MS\/MS) method was validated for the analysis of JWH\u2010018 in the specimens using deuterated \u0394<jats:sup>9<\/jats:sup>\u2010tetrahydrocannabinol (d<jats:sub>3<\/jats:sub>\u2010THC) as the internal standard. JWH\u2010018 was isolated by cold acetonitrile liquid\u2013liquid extraction. Chromatographic separation was performed on a Zorbaz eclipse XDB\u2010C<jats:sub>18<\/jats:sub> column. The assay was linear from 1 to 1000\u2009ng\/mL. Six C57BL6 mice were sacrificed 20\u2009min after exposure to the smoke of 200\u2009mg \u2018Buzz\u2019 containing 5.4% JWH\u2010018. Specimen concentrations of JWH\u2010018 were: blood, 54\u2013166\u2009ng\/mL (mean 82\u2009\u00b1\u200942\u2009ng\/mL); brain, 316\u2013708\u2009ng\/g (mean 510\u2009\u00b1\u2009166\u2009ng\/g); and liver, 1370\u20133220\u2009ng\/mL (mean 1990\u2009\u00b1\u2009752\u2009ng\/mL). The mean blood to brain ratio for JWH\u2010018 was 6.8 and ranged from 4.2 to 10.9. After exposure, the responses of the mice were consistent with cannabinoid receptor type 1 activity: body temperatures dropped 7.3\u2009\u00b1\u20091.1\u2009\u00b0C, and catalepsy, hyperreflexia, straub tail and ptosis were observed. The brain concentrations and physiological responses are consistent with the hypothesis that the behavioral effects of \u2018Buzz\u2019 are attributable to JWH\u2010018. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.2710","type":"journal-article","created":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T04:44:22Z","timestamp":1331181862000},"page":"1393-1398","source":"Crossref","is-referenced-by-count":16,"title":["Determination of naphthalen\u20101\u2010yl\u2010(1\u2010pentylindol\u20103\u2010yl)methanone (JWH\u2010018) in mouse blood and tissue after inhalation exposure to \u2018buzz\u2019 smoke by HPLC\/MS\/MS"],"prefix":"10.1002","volume":"26","author":[{"given":"Justin L.","family":"Poklis","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Virginia Commonwealth University  Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorra","family":"Amira","sequence":"additional","affiliation":[{"name":"Humphery Fellowship Program Virginia Commonwealth University  Richmond VA USA"},{"name":"D\u00e9partement Biologie Clinique \u2013 Toxicologie Universit\u00e9 de Monastir  Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura E.","family":"Wise","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Virginia Commonwealth University  Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason M.","family":"Wiebelhaus","sequence":"additional","affiliation":[{"name":"Department of Psychology Virginia Commonwealth University  Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenda J.","family":"Haggerty","sequence":"additional","affiliation":[{"name":"Department of Forensic Science Virginia Commonwealth University  Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aron H.","family":"Lichtman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Virginia Commonwealth University  Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alphonse","family":"Poklis","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Virginia Commonwealth University  Richmond VA USA"},{"name":"Department of Forensic Science Virginia Commonwealth University  Richmond VA USA"},{"name":"Department of Pathology Virginia Commonwealth University  Richmond VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/S0022-3565(24)37833-4","article-title":"Anandamide, an endogenous cannabinoid, has a very low physical dependence potential","volume":"287","author":"Aceto MD","year":"1998","journal-title":"Journal of Pharmacology and Experimental Therapy"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476\u20135381.2009.00582.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0376\u20138716(99)00152\u20130"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1558"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0022-3565(25)11216-0","article-title":"Pharmacological profile of a series of bicyclic cannabinoid analogs: classification as cannabimimetic agents","volume":"260","author":"Compton DR","year":"1992","journal-title":"Journal of Pharmacology and Experimental Therapy"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.161191698"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00428936"},{"key":"e_1_2_6_9_1","first-page":"1","article-title":"Scheduling update","volume":"44","author":"Drug Enforcement Administration","year":"2010","journal-title":"Microgram Bulletin"},{"key":"e_1_2_6_10_1","unstructured":"European Monitoring Centre for Drugs and Drug Addiction.Action on new drugs briefing paper: understanding the Spice phenomenon (a report from an EMCDDA expert meeting 6 March 2009 Lisbon) 2009. 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