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After solid\u2010phase extraction of plasma samples, imatinib and its internal standard, imatinib\u2010D8, were eluted with Zorbax SB\u2010C<jats:sub>18<\/jats:sub> at 60\u2009\u00b0C, under isocratic conditions through a mobile phase consisting of 4\u2009m<jats:sc>m<\/jats:sc> ammonium formate, pH: 3.2 (solution A) and acetonitrile solution B. The flow rate was 0.8\u2009mL\/min with 55% solution A\u2009+\u200945% solution B. Imatinib was detected and quantified by mass spectrometry with electrospray ionization operating in selected\u2010reaction monitoring mode. The calibration curve was linear in the range 10\u20135000\u2009ng\/mL, the lower limit of quantitation being 10\u2009ng\/mL. The method was validated according to the recommendations of the Food and Drug Administration, including tests of matrix effect (bias\u2009&lt;\u200910%) and recovery efficiency (&gt;80 and &lt;120%). 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