{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:39:09Z","timestamp":1790087949875,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,10,2]],"date-time":"2012-10-02T00:00:00Z","timestamp":1349136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Amoxicillin (AMO) degrades in plasma at room temperature and readily undergoes hydrolysis by the plasma amidase. In this paper, a novel, rapid and sensitive LC\u2010MS\/MS method operated in segmental and multiple reaction monitoring has been developed for the simultaneous determination of amoxicillin and ambroxol in human plasma. The degradation of amoxicillin in plasma was well prevented by immediate addition of 20 \u03bcL glacial acetic acid to 200 \u03bcL aliquot of freshly collected plasma samples before storage at \u221280\u00b0C. The sensitivity of the method was improved with segmental monitoring of the analytes, and lower limits of quantitation of 0.5 ng\/mL for ambroxol and 5 ng\/mL for amoxicillin were obtained. The sensitivity of our method was five times better than those of the existing methods. Furthermore, the mass response saturation problem with amoxicillin was avoided by diluting the deproteinized plasma samples with water before injection into the LC\u2010MS\/MS system. The method was successfully employed in a pharmacokinetic study of the compound amoxicillin and ambroxol hydrochloride tablets. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.2824","type":"journal-article","created":{"date-parts":[[2012,10,2]],"date-time":"2012-10-02T07:41:33Z","timestamp":1349163693000},"page":"520-526","source":"Crossref","is-referenced-by-count":32,"title":["A sensitive LC\u2010MS\/MS method for the simultaneous determination of amoxicillin and ambroxol in human plasma with segmental monitoring"],"prefix":"10.1002","volume":"27","author":[{"given":"Xin","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis China Pharmaceutical University  24 Tongjiaxiang Nanjing 210009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis China Pharmaceutical University  24 Tongjiaxiang Nanjing 210009 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