{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T08:58:54Z","timestamp":1780477134499,"version":"3.54.1"},"reference-count":13,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T00:00:00Z","timestamp":1353628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A selective, sensitive and rapid high\u2010performance liquid chromatography method with post\u2010column hydrolysis and fluorescence detection was developed for the simultaneous quantification of acetylsalicylic acid and its metabolite salicylic acid in human plasma. Following the addition of 2\u2010hydroxy\u20103\u2010methoxybenzoic acid as internal standard and simple protein precipitation with acetonitrile, the analytes were separated on a ProntoSIL 120 C<jats:sub>18<\/jats:sub> ace\u2010EPS column (150\u2009\u00d7\u20092\u2009mm, 3\u2009\u00b5m) protected by a C<jats:sub>8<\/jats:sub> guard column (5\u2009\u00b5m). The mobile phase, 10\u2009m<jats:sc>m<\/jats:sc> formic acid in water (pH 2.9) and acetonitrile (70:30, v\/v), was used at a flow rate of 0.35\u2009mL\/min. After on\u2010line post\u2010column hydrolysis of acetylsalicylic acid (ASA) to salicylic acid (SA) by addition of alkaline solution, the analytes were measured at 290\u2009nm (<jats:italic>\u03bb<\/jats:italic><jats:sub>ex<\/jats:sub>) and 400\u2009nm (<jats:italic>\u03bb<\/jats:italic><jats:sub>em<\/jats:sub>). The method was linear in the concentration ranges between 0.05 and 20\u2009ng\/\u03bcL for both ASA and SA with a lower limit of quantification of 25\u2009pg\/\u03bcL for SA and 50\u2009pg\/\u03bcL for ASA. The limit of detection was 15\u2009pg\/\u03bcL for SA and 32.5\u2009pg\/\u03bcL for ASA. The analysis of ASA and SA can be carried out within 8\u2009min; therefore this method is suitable for measuring plasma concentrations of salicylates in clinical routine. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.2846","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T02:51:33Z","timestamp":1353639093000},"page":"695-698","source":"Crossref","is-referenced-by-count":14,"title":["Simultaneous determination of acetylsalicylic acid and salicylic acid in human plasma by isocratic high\u2010pressure liquid chromatography with post\u2010column hydrolysis and fluorescence detection"],"prefix":"10.1002","volume":"27","author":[{"given":"Eva\u2010Luise","family":"Hobl","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Jilma","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Ebner","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer W.","family":"Schmid","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.973"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600720913"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1093\/clinchem\/28.5.1200","article-title":"Improved liquid\u2010chromatography of aspirin, salicylate, and salicyluric acid in plasma, with a modification for determining aspirin metabolites in urine","volume":"28","author":"Buskin JN","year":"1982","journal-title":"Clinical Chemistry"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-198706000-00020"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(98)00081-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600691109"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(95)00464-5"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)84723-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9707040"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.2694"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199405053301808"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600670540"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80200-1"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.2846","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.2846","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:15:22Z","timestamp":1760040922000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.2846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,23]]},"references-count":13,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1002\/bmc.2846"],"URL":"https:\/\/doi.org\/10.1002\/bmc.2846","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,23]]}}}