{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T20:39:47Z","timestamp":1778791187863,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,6,11]],"date-time":"2013-06-11T00:00:00Z","timestamp":1370908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Bacteriocins, an antimicrobial peptide, is known to have wide spectrum antimicrobial activity against various pathogens. Because they are easily digested in the intestine, they are considered as safe and are widely used as food preservatives. Hence their purification and characterization have attracted considerable attraction, especially for those having activity against human pathogens. In this study, the bacteriocin produced by <jats:italic>Pediococcus pentosaceus<\/jats:italic> VJ13 was precipitated with cold acetone and purified by gel permeation chromatography and hydrophobic interaction chromatography. The bacteriocin exhibited antimicrobial activity against various pathogens, like <jats:italic>Mycobacterium smegmatis<\/jats:italic>, <jats:italic>Klebsiella pneumonia<\/jats:italic>, <jats:italic>Clostridium perfringens<\/jats:italic> and <jats:italic>Staphylococcus epidermidis<\/jats:italic>. The activity of bacteriocin was lost completely after treatment with protease, which revealed its proteinaceous nature. The bacteriocin was stable up to 100\u00b0C and exhibited antilisterial property which is a characteristic feature of class IIa bacteriocins. It was active within the pH range of 2\u20138 and stable against various chemicals and denaturants. Tricine SDS\u2013PAGE revealed its molecular weight to be 4.0 kDa, where the corresponding activity against <jats:italic>Listeria monocytogenes<\/jats:italic> was also noted. Treatment of <jats:italic>L<\/jats:italic>. <jats:italic>monocytogenes<\/jats:italic> with bacteriocin decreased the viable cell count, and scanning electron microscope analysis revealed membrane pore formation that resulted in the release of intracellular content, suggesting its bactericidal effect. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.2948","type":"journal-article","created":{"date-parts":[[2013,6,11]],"date-time":"2013-06-11T04:53:22Z","timestamp":1370926402000},"page":"1497-1502","source":"Crossref","is-referenced-by-count":29,"title":["Bacteriocin activity against various pathogens produced by <i>Pediococcus pentosaceus<\/i> VJ13 isolated from <i>Idly<\/i> batter"],"prefix":"10.1002","volume":"27","author":[{"given":"Venkatasubramanian","family":"Vidhyasagar","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Pondicherry University  R.V. Nagar Kalapet Puducherry 605014 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kadirvelu","family":"Jeevaratnam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Pondicherry University  R.V. Nagar Kalapet Puducherry 605014 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2010.11.029"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.01.011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-011-0743-4"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01569434"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1991.tb03782.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05576-11"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2007.01.019"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2010.07.010"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213-010-0145-x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.10.021"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-004-1576-8"},{"key":"e_1_2_7_13_1","first-page":"446","article-title":"Biological preservation of foods \u2013 bacteriocins of lactic aicd bacteria","volume":"4","author":"Jeevaratnam K","year":"2005","journal-title":"Indian Journal of Biotechnology"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2011.07.027"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1110.10002"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1993.tb00012.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-011-0971-4"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2010.05.005"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920109787048661"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-8-3"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7012(94)00068-I"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408690290825475"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90587-2"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2007.11.024"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213-012-0486-8"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.04.010"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2010.05.001"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2012.10.012"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.13005\/bbra\/1019"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-010-0541-1"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.2948","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.2948","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:43:19Z","timestamp":1760056999000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.2948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,11]]},"references-count":29,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1002\/bmc.2948"],"URL":"https:\/\/doi.org\/10.1002\/bmc.2948","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,11]]}}}