{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T03:21:22Z","timestamp":1770348082867,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,7,23]],"date-time":"2013-07-23T00:00:00Z","timestamp":1374537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We report a qualitative liquid chromatography\u2013tandem mass spectrometry (LC\/MS\/MS) method for the simultaneous analysis of the three known <jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>\u2010dimethyltryptamine endogenous hallucinogens, their precursors and metabolites, as well as melatonin and its metabolic precursors. The method was characterized using artificial cerebrospinal fluid (aCSF) as the matrix and was subsequently applied to the analysis of rat brain pineal gland\u2010aCSF microdialysate. The method describes the simultaneous analysis of 23 chemically diverse compounds plus a deuterated internal standard by direct injection, requiring no dilution or extraction of the samples. The results demonstrate that this is a simple, sensitive, specific and direct approach to the qualitative analysis of these compounds in this matrix. The protocol also employs stringent MS confirmatory criteria for the detection and confirmation of the compounds examined, including exact mass measurements. The excellent limits of detection and broad scope make it a valuable research tool for examining the endogenous hallucinogen pathways in the central nervous system. We report here, for the first time, the presence of <jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>\u2010dimethyltryptamine in pineal gland microdialysate obtained from the rat. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.2981","type":"journal-article","created":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T04:07:53Z","timestamp":1383624473000},"page":"1690-1700","source":"Crossref","is-referenced-by-count":61,"title":["LC\/MS\/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate"],"prefix":"10.1002","volume":"27","author":[{"given":"Steven A.","family":"Barker","sequence":"first","affiliation":[{"name":"Department of Comparative Biomedical Sciences, School of Veterinary Medicine Louisiana State University  Baton Rouge LA 70803 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jimo","family":"Borjigin","sequence":"additional","affiliation":[{"name":"Departments of Molecular &amp; Integrative Physiology<sup>1<\/sup> and Neurology<sup>2<\/sup> University of Michigan Medical School  Ann Arbor MI 48109 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izabela","family":"Lomnicka","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedical Sciences, School of Veterinary Medicine Louisiana State University  Baton Rouge LA 70803 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rick","family":"Strassman","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of New Mexico School of Medicine, Albuquerque, and Cottonwood Research Foundation  Gallup New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,23]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"113","volume-title":"The Symposium on Beta\u2010Carbolines and Tetrahydro\u2010isoquinolines","author":"Barker SA","year":"1982"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(79)91659-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(80)90169-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(81)90278-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(08)60291-3"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(84)90404-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.422"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(84)90007-1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2007.10.010"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.08.004"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142301.ns0701s47"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1192\/bjp.132.2.139"},{"key":"e_1_2_6_14_1","first-page":"840.19","article-title":"Indolethylamine N\u2010methyltransferase expression in primate nervous tissue","volume":"37","author":"Cozzi NV","year":"2011","journal-title":"Society for Neuroscience Abstracts"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(55)90150-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1166127"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2013.02.004"},{"key":"e_1_2_6_18_1","first-page":"136","article-title":"1,2,3,4\u2010Tetrahydro\u2010\u03b2\u2010carboline","volume":"51","author":"Ho BT","year":"1964","journal-title":"Organic Synthesis"},{"key":"e_1_2_6_19_1","first-page":"19","article-title":"Mass spectrometric identification of 6\u2010methoxy\u20101,2,3,4\u2010tetrahydro\u2010\u03b2\u2010carboline in pineal gland. 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