{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:32:36Z","timestamp":1790087556418,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T00:00:00Z","timestamp":1374710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2013,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n A simple, sensitive and specific ultra\u2010performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2010MS\/MS) method was developed and validated to determine the concentrations of 7\u2010hydroxymitragynine in rat plasma. Following a single\u2010step liquid\u2013liquid extraction of plasma samples using chloroform, 7\u2010hydroxymitragynine and the internal standard (tryptoline) were separated on an Acquity UPLC\n TM<\/jats:sup>\n BEH C\n 18<\/jats:sub>\n (1.7\u2009\u00b5m, 2.1\u2009\u00d7\u200950\u2009mm) column using an isocratic elution at a flow rate of 0.2\u2009mL\/min. The mobile phase consisted of 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile (10:90, v\/v). The run time was 2.5\u2009min. The analysis was carried out under the multiple reaction\u2010monitoring mode using positive electrospray ionization. Protonated ions [M\u2009+\u2009H]\n +<\/jats:sup>\n and their respective product ions were monitored at the following transitions: 415\u2009\u2192\u2009190 for 7\u2010hydroxymitragynine and 173\u2009\u2192\u2009144 for the internal standard. The calibration curve was linear over the range of 10\u20134000\u2009ng\/mL (\n r<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.999) with a lower limit of quantification of 10\u2009ng\/mL. The extraction recoveries ranged from 62.0 to 67.3% at concentrations of 20, 600 and 3200\u2009ng\/mL). Intra\u2010 and inter\u2010day assay precisions (relative standard deviation) were <15% and the accuracy was within 96.5\u2013104.0%. This validated method was successfully applied to quantify 7\u2010hydroxymitragynine in rat plasma following intravenous administration. Copyright \u00a9 2013 John Wiley & Sons, Ltd.\n <\/jats:p>","DOI":"10.1002\/bmc.2986","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T11:26:26Z","timestamp":1374751586000},"page":"1726-1732","source":"Crossref","is-referenced-by-count":17,"title":["Development and validation of a UPLC\u2010MS\/MS method for the determination of 7\u2010hydroxymitragynine, a\n \u03bc<\/i>\n \u2010opioid agonist, in rat plasma and its application to a pharmacokinetic study"],"prefix":"10.1002","volume":"27","author":[{"given":"Pradeep K.","family":"Vuppala","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy The University of Mississippi University MS 38677 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seshulatha","family":"Jamalapuram","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy The University of Mississippi University MS 38677 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward B.","family":"Furr","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, School of Pharmacy The University of Mississippi University MS 38677 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher R.","family":"McCurdy","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, School of Pharmacy The University of Mississippi University MS 38677 USA"},{"name":"Department of Pharmacology, School of Pharmacy The University of Mississippi University MS 38677 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonnie A.","family":"Avery","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy The University of Mississippi University MS 38677 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/156802611795371305"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15563650701241795"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.05.016"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706435"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-837822"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.12.013"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.2901"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11419-009-0070-5"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bks073"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1177\/1934578X0900400705","article-title":"Phytochemical characterization of the leaves of Mitragyna speciosa grown in U.S.A","volume":"4","author":"Leon F","year":"2009","journal-title":"Natural Product Communications"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-012-1103-8"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2003.09.054"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.10.086"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.08.013"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2008.05.003"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2005.06.035"},{"key":"e_1_2_7_18_1","first-page":"19","article-title":"Principles and applications of liquid chromatography\u2013mass spectrometry in clinical biochemistry","volume":"30","author":"Pitt JJ","year":"2009","journal-title":"The Clinical Biochemist. 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