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The limits of detection for 4\u2010(methylnitrosamino)\u20101\u2010(3\u2010pyridyl)\u20101\u2010butanol, <jats:italic>N<\/jats:italic>\u2032\u2010nitrosonornicotine, 4\u2010(methylnitrosamino)\u20101\u2010(3\u2010pyridyl)\u20101\u2010butanone, <jats:italic>N<\/jats:italic>\u2032\u2010nitrosoanatabine and <jats:italic>N<\/jats:italic>\u2032\u2010nitrosoanabasine were 0.6, 0.6, 10.0, 0.4 and 0.4 pg\/mL, respectively, with a linear calibration range of up to 20,000 pg\/mL. Intra\u2010 and inter\u2010day precision for TSNA measurements ranged from 0.82 to 3.67% and from 2.04 to 7.73% respectively. For total TSNAs, the <jats:italic>\u03b2<\/jats:italic>\u2010glucuronidase amount was optimized for hydrolysis time and yield. Different liquid chromatography columns and mobile phases with different pH conditions were evaluated. The validated method was then applied to 50 smoker and 30 nonsmoker urine samples. Our results suggest that this sensitive and relatively simple analytical method is suitable for application to epidemiological investigations of health risks associated with the exposure to tobacco smoke or secondhand smoke in both smokers and nonsmokers. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.<\/jats:p>","DOI":"10.1002\/bmc.3031","type":"journal-article","created":{"date-parts":[[2013,10,14]],"date-time":"2013-10-14T07:37:12Z","timestamp":1381736232000},"page":"375-384","source":"Crossref","is-referenced-by-count":40,"title":["Quantitative analysis of five tobacco\u2010specific <i>N<\/i>\u2010nitrosamines in urine by liquid chromatography\u2013atmospheric pressure ionization tandem mass spectrometry"],"prefix":"10.1002","volume":"28","author":[{"given":"Baoyun","family":"Xia","sequence":"first","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health, Centers for Disease Control and Prevention  4770 Buford Highway NE, Atlanta GA 30341 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Xia","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health, Centers for Disease Control and Prevention  4770 Buford Highway NE, Atlanta GA 30341 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Wong","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health, Centers for Disease Control and Prevention  4770 Buford Highway NE, Atlanta GA 30341 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keegan J.","family":"Nicodemus","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health, Centers for Disease Control and Prevention  4770 Buford Highway NE, Atlanta GA 30341 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health, Centers for Disease Control and Prevention  4770 Buford Highway NE, Atlanta GA 30341 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health, Centers for Disease Control and Prevention  4770 Buford Highway NE, Atlanta GA 30341 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonya","family":"Guillot","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health, Centers for Disease Control and Prevention  4770 Buford Highway NE, Atlanta GA 30341 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences National Center for Environmental Health, Centers for Disease Control and Prevention  4770 Buford Highway NE, Atlanta GA 30341 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,10,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/31.8.477"},{"key":"e_1_2_7_3_1","unstructured":"Agilent.1200 Infinity Calculator 2013. 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