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Previous publications on chromatographic methods of CsA determination in ocular tissues involved only reversed\u2010phase HPLC separation, usually in combination with such detection techniques as radio\u2010immunoassay and mass spectrometry. The application of the UHPLC technique allowed us to significantly decrease the analysis time. Cyclosporine D (CsD) was applied as the internal standard. Satisfactory separation was achieved on an XB\u2010C<jats:sub>18<\/jats:sub> Kinetex column at 60\u00b0C with the use of gradient elution mode. The retention times of CsA and CsD were found to be 4.5 and 5.1 min, respectively. The developed assay is specific, sensitive (limit of detection = 6 ng\/mL and limit of quantitation = 18 ng\/mL) and linear within the analyte concentration range of 0.018\u20135 \u00b5g\/mL, with a correlation coefficient of 0.999. High sensitivity, low injection volume (10 \u03bcL), short time of analysis (6.5 min) and simplicity make this method useful for the fast analysis of CsA in rabbit ocular tissues and fluids: lacrimal fluid, aqueous humor, cornea, conjunctiva and eye globe. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3132","type":"journal-article","created":{"date-parts":[[2014,5,25]],"date-time":"2014-05-25T20:36:54Z","timestamp":1401050214000},"page":"802-809","source":"Crossref","is-referenced-by-count":12,"title":["Development and validation of UHPLC method for the determination of cyclosporine A in biological samples"],"prefix":"10.1002","volume":"28","author":[{"given":"Oliwia","family":"Szerkus","sequence":"first","affiliation":[{"name":"Department of Biopharmaceutics and Pharmacodynamics Medical University of Gda\u0144sk  Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliza","family":"Wolska","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology Medical University of Gda\u0144sk  Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wiktoria","family":"Struck\u2010Lewicka","sequence":"additional","affiliation":[{"name":"Department of 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