{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T03:12:50Z","timestamp":1761621170517,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T00:00:00Z","timestamp":1398988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A simple, rapid, specific and reliable high\u2010performance liquid chromatographic assay of meloxicam in human plasma has been developed using a C<jats:sub>18<\/jats:sub> reversed\u2010phase analytical column. Reversed\u2010phase chromatography was conducted using a mobile phase of 0.02 potassium dihydrogen phosphate (adjusted to pH\u20092.7 with phosphoric acid)\u2013acetonitrile\u2013triethylamine (35:65:0.05, v\/v) with UV detection at 354\u2009nm. The drug in human plasma was deproteinized using a combination of methanol and chloroform. This method is simple, rapid and consistent with a high recovery of meloxicam in human plasma ranging from 93.29 to 111.09%. Regression analysis for the calibration plot for plasma standards obtained for the drug concentrations between (25\u20134000) ng\/mL indicated excellent linearity (<jats:italic>r<\/jats:italic>\u2009\u2265\u20090.9997). The proposed method was applied to study the bioequivalence between Mobic (original) and Melocam (generic) products. The study was conducted on using two tablets (4\u2009\u00d7\u20097.5\u2009mg) of each of the commercial product and the reference standard in a two\u2010way open randomized crossover design involving 20 volunteers. Area under the concentration\u2013time curve, peak concentration (<jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub>) and time to reach <jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub> were 72,868.61\u2009ng\u2009h\/mL, 2133.93\u2009ng\/mL and 4.06\u2009h for Mobic, and 78,352.52\u2009ng\u2009h\/mL, 2525.18\u2009ng\/mL and 3.61\u2009h for Melocam. Two <jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub> were discovered in the pharmacokinetic profiles which confirm enterohepatic recirculation. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3221","type":"journal-article","created":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T04:05:24Z","timestamp":1399003524000},"page":"1782-1788","source":"Crossref","is-referenced-by-count":5,"title":["Improved protein deproteinization method for the determination of meloxicam in human plasma and application in pharmacokinetic study"],"prefix":"10.1002","volume":"28","author":[{"given":"Kai Bin","family":"Liew","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Universiti Sains Malaysia  11800 Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Onn Kit","family":"Loh","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Universiti Sains Malaysia  11800 Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne Tze Fung","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Universiti Sains Malaysia  11800 Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kok Khiang","family":"Peh","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Universiti Sains Malaysia  11800 Minden Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,2]]},"reference":[{"issue":"4","key":"e_1_2_7_2_1","first-page":"739","article-title":"Meloxicam in rheumatoid arthritis","volume":"1","author":"Ahmed M","year":"2005","journal-title":"Drug Evaluation"},{"key":"e_1_2_7_3_1","first-page":"266","article-title":"Bioequivalence between two metronidazole formulations","volume":"26","author":"Alves AJ","year":"2007","journal-title":"Latin American Journal of Pharmacy"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91782-9"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/004982598239704"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199936020-00003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1081\/AL-100104963"},{"issue":"2","key":"e_1_2_7_8_1","first-page":"115","article-title":"Validation of the analytical method for in\u2010vivo determination of meloxicam and bioequivalence study from meloxicam containing microparticle formulations in rabbits","volume":"29","author":"Ero\u011flu H","year":"2009","journal-title":"Hacettepe University Journal of the Faculty of Pharmacy"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91378-5"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2012.01402.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2165\/11313370-000000000-00000"},{"issue":"1","key":"e_1_2_7_12_1","first-page":"115","article-title":"Bioequivalence study of two brands of meloxicam tablets in healthy human Pakistani male subjects","volume":"68","author":"Mahmood A","year":"2011","journal-title":"Acta Poloniae Pharmaceutica, Drug Research"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.446"},{"volume-title":"The Complete Drug Reference","year":"2008","author":"Martindale","key":"e_1_2_7_14_1"},{"issue":"6","key":"e_1_2_7_15_1","first-page":"326","article-title":"A non\u2010extracting procedure for the determination of meloxicam in plasma samples by HPLC\u2010diode array detection","volume":"55","author":"Medvedovici A","year":"2005","journal-title":"Arzneimittel\u2010Forschung"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.5414\/CPP44489"},{"issue":"11","key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1016\/S0090-9556(25)06817-5","article-title":"Meloxicam pharmacokinetics and metabolic pattern after intravenous infusion and oral administration to healthy volunteers","volume":"23","author":"Schmid J","year":"1995","journal-title":"Drug Metabolism and Disposition"},{"issue":"3","key":"e_1_2_7_18_1","first-page":"253","article-title":"Clinical pharmacokinetics of meloxicam","volume":"47","author":"Turck D","year":"1997","journal-title":"Arzneimittel\u2010Forschung"},{"key":"e_1_2_7_19_1","unstructured":"US Food and Drug Administration.Guidance for Industry: Bioanalytical Method Validation. US Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research: Rockville MD 2001. Available from:http:\/\/www.fda.gov\/cder\/guidance\/index\/htm(accessed 28 July 2010)."},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91782-9"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3221","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.3221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:50:13Z","timestamp":1760050213000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.3221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,2]]},"references-count":19,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1002\/bmc.3221"],"URL":"https:\/\/doi.org\/10.1002\/bmc.3221","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2014,5,2]]}}}