{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:10:28Z","timestamp":1760141428284,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,7,3]],"date-time":"2014-07-03T00:00:00Z","timestamp":1404345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sialation of cell surface is known to be tightly connected with tumorigenicity, invasiveness, metastatic potential and clearance of aged cells, while sialation of immunoglobulin G (IgG) molecules determines their anti\u2010inflammatory properties. Recently, we have found for the first time IgG\u2010antibodies possessing sialidase\u2010like activity (sialylic abzyme) in blood serum of multiple myeloma and systemic lupus erythematosis patients. This abzyme was detected in a pool of IgGs purified by a typical procedure including immunoglobulin's precipitation with ammonium sulfate and following chromatography on protein G\u2013Sepharose column. Here we describe a novel matrix for affinity purification of sialylic abzyme that is based on using bovine submandibular gland mucin conjugated to Sepharose matrix (mucin\u2013Sepharose). This matrix preferentially binds sialidase\u2010like IgGs from a pool of sialidase\u2010active fraction of proteins precipitated with 50% ammonium sulfate from blood serum of the systemic lupus erythematosis patients. That allowed us to develop a new scheme of double\u2010step chromatography purification of sialidase\u2010like IgGs from human blood serum<jats:italic>.<\/jats:italic> Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3283","type":"journal-article","created":{"date-parts":[[2014,7,3]],"date-time":"2014-07-03T05:11:49Z","timestamp":1404364309000},"page":"328-332","source":"Crossref","is-referenced-by-count":1,"title":["Two\u2010step chromatography purification of IgGs possessing sialidase activity from human blood serum"],"prefix":"10.1002","volume":"29","author":[{"given":"Yury","family":"Kit","sequence":"first","affiliation":[{"name":"Institute of Cell Biology National Academy of Sciences of Ukraine  Drahomanov St., 14\/16 79005 Lviv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rostyslav","family":"Bilyy","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Sciences of Ukraine  Drahomanov St., 14\/16 79005 Lviv Ukraine"},{"name":"Danylo Halytsky Lviv National Medical University  Pekarska St., 69 79010 Lviv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nataliya","family":"Korniy","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Sciences of Ukraine  Drahomanov St., 14\/16 79005 Lviv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andriy","family":"Tomin","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Sciences of Ukraine  Drahomanov St., 14\/16 79005 Lviv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentyna","family":"Chop'yak","sequence":"additional","affiliation":[{"name":"Danylo Halytsky Lviv National Medical University  Pekarska St., 69 79010 Lviv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaroslav","family":"Tolstyak","sequence":"additional","affiliation":[{"name":"Danylo Halytsky Lviv National Medical University  Pekarska St., 69 79010 Lviv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volodymyr","family":"Antonyuk","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Sciences of Ukraine  Drahomanov St., 14\/16 79005 Lviv Ukraine"},{"name":"Danylo Halytsky Lviv National Medical University  Pekarska St., 69 79010 Lviv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rostyslav","family":"Stoika","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Sciences of Ukraine  Drahomanov St., 14\/16 79005 Lviv Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200900020"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.1071"},{"key":"e_1_2_6_4_1","first-page":"89","volume-title":"Autoimmune Disorders \u2013 Pathogenetic Aspects","author":"Bilyy R","year":"2011"},{"key":"e_1_2_6_5_1","unstructured":"BilyyRO BilaEEandKitYY.Catalytic antibodies and uses thereof. 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