{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:34:40Z","timestamp":1786977280961,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T00:00:00Z","timestamp":1404691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A rapid, accurate and sensitive thin\u2010layer chromatography (TLC) method with densitometric detection has been developed and validated for the determination of cefepime in pharmaceuticals. Chromatographic separation was achieved on a silica gel TLC F\n                    <jats:sub>254<\/jats:sub>\n                    plates with a mobile phase consisting of ethanol\u20132\u2010propanol\u2013glacial acetic acid 99.5%\u2013water (4:4:1:3, v\/v). Densitometric detection was carried out at wavelength of 266\u2009nm in reflectance\/absorbance mode. The validation of the method was found to be satisfactory with high accuracy (from 99.24 to 101.37%) and precision (RSD from 0.06 to 0.36%). Additionally, the stability of cefepime in solution was investigated, including the effect of pH, temperature and incubation time. Favorable retention parameters (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>f<\/jats:sub>\n                    ,\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>s,<\/jats:sub>\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ) were obtained under the developed conditions, which guaranteed good separation of the studied components. The degradation process of cefepime hydrochloride was described by kinetic and thermodynamic parameters (\n                    <jats:italic>k<\/jats:italic>\n                    ,\n                    <jats:italic>t<\/jats:italic>\n                    <jats:sub>0.1<\/jats:sub>\n                    ,\n                    <jats:italic>t<\/jats:italic>\n                    <jats:sub>0.5<\/jats:sub>\n                    and\n                    <jats:italic>E<\/jats:italic>\n                    <jats:sub>a<\/jats:sub>\n                    ). Moreover, the chemical properties of degradation products were characterized by the\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>f<\/jats:sub>\n                    values, absorption spectra, HPLC\u2010MS\/MS and TLC\u2010densitometry analysis. As the method could effectively separate the active substance from its main degradation product (1\u2010methylpyrrolidine), it can be employed as a method to indicate the stability of this drug. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/bmc.3288","type":"journal-article","created":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T03:41:24Z","timestamp":1404704484000},"page":"388-395","source":"Crossref","is-referenced-by-count":13,"title":["A degradation study of cefepime hydrochloride in solutions under various stress conditions by TLC\u2013densitometry"],"prefix":"10.1002","volume":"29","author":[{"given":"Monika","family":"D\u0105browska","sequence":"first","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Collegium Medicum Jagiellonian University  9 Medyczna Str 30\u2010688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Starek","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Collegium Medicum Jagiellonian University  9 Medyczna Str 30\u2010688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Krzek","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Collegium Medicum Jagiellonian University  9 Medyczna Str 30\u2010688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u017cbieta","family":"Papp","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Collegium Medicum Jagiellonian University  9 Medyczna Str 30\u2010688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Kr\u00f3l","sequence":"additional","affiliation":[{"name":"Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Collegium Medicum Jagiellonian University  9 Medyczna Str 30\u2010688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg134"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00343-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(96)01796-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0801(199906)13:4<272::AID-BMC842>3.0.CO;2-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)01207-3"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(03)00307-8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00630-7"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00406-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(02)00653-2"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-008-0529-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/js980170y"},{"key":"e_1_2_6_13_1","first-page":"1613","article-title":"Spectrophotometric determination of ezetimibe and cefepime","volume":"19","author":"Gowri Sankar D","year":"2007","journal-title":"Asian Journal of Chemistry"},{"key":"e_1_2_6_14_1","unstructured":"ICH.Harmonized Tripartite Guideline Q2(R1) on \u2018Validation of Analytical Procedures: Text and Methodology\u2019. 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