{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:01:32Z","timestamp":1791000092750,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,8,4]],"date-time":"2014-08-04T00:00:00Z","timestamp":1407110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81001468"],"award-info":[{"award-number":["81001468"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Prescription and nonprescription products for topical management of pain, including cream, lotion and patch forms, contain capsaicin (CAP) and dihydrocapsaicin (DHC). There are few\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies on absorption, bioavailability and disposition of CAP and DHC. We established a sensitive and rapid LC\u2010MS\/MS assay to determine CAP and DHC levels in rabbit plasma and tissue. Bio\u2010samples prepared by liquid\u2013liquid extraction using\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane\u2013dichloromethane\u2013isopropanol (100: 50: 5, v\/v\/v) mixture were separated by isocratic chromatography with an Extend C\n                    <jats:sub>18<\/jats:sub>\n                    column. The mobile phase was acetonitrile\u2013water\u2013formic acid (70: 30: 0.1, v\/v\/v). The method was linear from 0.125 to 50 ng\/mL for a 100 \u03bcL bio\u2010sample, and the lower quantification limit was 0.125 ng\/mL. Total run time to analyze each sample was 3.5 min. We used this validated method to study pharmacokinetics and tissue distribution of CAP gel administered topically to rabbits. A very small amount of CAP and DHC was absorbed into the systemic circulation. The highest plasma concentration was 2.39 ng\/mL, and the mean peak plasma concentration value after 12 h of CAP gel application was 1.68 ng\/mL. Drug concentration in treated skin was relatively high, with low concentration in other tissues. Thus, topical CAP gel had strong local effects and weaker systemic effects. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/bmc.3302","type":"journal-article","created":{"date-parts":[[2014,8,4]],"date-time":"2014-08-04T05:13:04Z","timestamp":1407129184000},"page":"496-503","source":"Crossref","is-referenced-by-count":17,"title":["A sensitive LC\u2010MS\/MS method for quantifying capsaicin and dihydrocapsaicin in rabbit plasma and tissue: application to a pharmacokinetic study"],"prefix":"10.1002","volume":"29","author":[{"given":"Dimin","family":"Wang","sequence":"first","affiliation":[{"name":"Shenyang Pharmaceutical University  103 Wenhua Road Shenyang 110016 People's Republic of China"},{"name":"Beijing Institute of Pharmacology and Toxicology  27 Taiping Road Beijing 100850 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanhua","family":"Meng","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  27 Taiping Road Beijing 100850 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yu","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  103 Wenhua Road Shenyang 110016 People's Republic of China"},{"name":"Beijing Institute of Pharmacology and Toxicology  27 Taiping Road Beijing 100850 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu.","family":"Sun","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University  103 Wenhua Road Shenyang 110016 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Sun","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  27 Taiping Road Beijing 100850 People's Republic of China"},{"name":"Shenyang University of Chemical Technology  Shenyang 110142 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jifen","family":"Guo","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology  27 Taiping Road Beijing 100850 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e3181a8b200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1107"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.13.11.1445"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.2004.04082.x"},{"key":"e_1_2_7_6_1","unstructured":"FDA.Guidance for industry \u2013 bioanalytical methods validation 2011. Available at:http:\/\/www.fda.gov\/downloads\/Drugs\/GuidanceComplianceRegulatoryInformation\/Guidances\/ucm070107.pdf(accessed 4 June 2011)."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.01.016"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac020361s"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/26.6.313"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16021253"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-6997(24)01403-0"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-005-3102-y"},{"key":"e_1_2_7_13_1","first-page":"143","article-title":"Structure and function of TRPV1. 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