{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:53:23Z","timestamp":1760061203826,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,8,19]],"date-time":"2014-08-19T00:00:00Z","timestamp":1408406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple and specific bioanalytical method based on reversed\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC) coupled with ultraviolet detection was developed and validated for the determination of a novel valproic acid arylamide, <jats:italic>N<\/jats:italic>\u2010(2\u2010hydroxyphenyl)\u20102\u2010propylpentanamide (HO\u2010AAVPA) in rat hepatic microsomes (a subcellular fraction containing phase I enzymes, especially cytochrome P450). The chromatographic separation was achieved using a reversed\u2010phase Zorbax SB\u2010C<jats:sub>18<\/jats:sub> column and a mobile phase of acetic acid in water (0.2% v\/v) and acetonitrile (40:60\u2009v\/v) with a flow rate of 0.5\u2009mL\/min. The calibration curve was linear over the range of 882\u20137060\u2009ng\/mL (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.9987), and the lower limit of quantification and the lower limit of determination were found to be 882 and 127.99\u2009ng\/mL, respectively. The method was validated with excellent sensitivity, and intra\u2010day accuracy and precision varied from 93.79 to 93.12%, and from 2.12 to 4.36%, respectively. The inter\u2010day accuracy and precision ranged from 93.29 to 97.30% and from 0.68 to 3.60%, respectively. The recovery of HO\u2010AAVPA was measured between 91.36 and 97.98%. The assay was successfully applied to the analysis of kinetic metabolism and pharmacokinetic parameters <jats:italic>in vitro<\/jats:italic> by a substrate depletion approach. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3307","type":"journal-article","created":{"date-parts":[[2014,8,19]],"date-time":"2014-08-19T01:14:59Z","timestamp":1408410899000},"page":"523-528","source":"Crossref","is-referenced-by-count":9,"title":["A simple validated RP\u2010HPLC bioanalytical method for the quantitative determination of a novel valproic acid arylamide derivative in rat hepatic microsomes"],"prefix":"10.1002","volume":"29","author":[{"given":"Arianna","family":"Silva\u2010Trujillo","sequence":"first","affiliation":[{"name":"Laboratorio de Biof\u00edsica y Biocat\u00e1lisis y Laboratorio de Modelado Molecular y Dise\u00f1o de F\u00e1rmacos de la Escuela Superior de Medicina Instituto Polit\u00e9cnico Nacional  Plan de San Luis y D\u00edaz Mir\u00f3n s\/n, Casco de Santo Tom\u00e1s Distrito Federal 11340 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Correa\u2010Basurto","sequence":"additional","affiliation":[{"name":"Laboratorio de Biof\u00edsica y Biocat\u00e1lisis y Laboratorio de Modelado Molecular y Dise\u00f1o de F\u00e1rmacos de la Escuela Superior de Medicina Instituto Polit\u00e9cnico Nacional  Plan de San Luis y D\u00edaz Mir\u00f3n s\/n, Casco de Santo Tom\u00e1s Distrito Federal 11340 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurelio","family":"Romero\u2010Castro","sequence":"additional","affiliation":[{"name":"Laboratorio de Biof\u00edsica y Biocat\u00e1lisis y Laboratorio de Modelado Molecular y Dise\u00f1o de F\u00e1rmacos de la Escuela Superior de Medicina Instituto Polit\u00e9cnico Nacional  Plan de San Luis y D\u00edaz Mir\u00f3n s\/n, Casco de Santo Tom\u00e1s Distrito Federal 11340 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnulfo","family":"Albores","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Toxicolog\u00eda Cinvestav\u2010IPN  M\u00e9xico City D.F. 07360 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica Elena","family":"Mendieta\u2010Wejebe","sequence":"additional","affiliation":[{"name":"Laboratorio de Biof\u00edsica y Biocat\u00e1lisis y Laboratorio de Modelado Molecular y Dise\u00f1o de F\u00e1rmacos de la Escuela Superior de Medicina Instituto Polit\u00e9cnico Nacional  Plan de San Luis y D\u00edaz Mir\u00f3n s\/n, Casco de Santo Tom\u00e1s Distrito Federal 11340 Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,8,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seizure.2011.10.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0461(1999)13:1<53::AID-JBT7>3.0.CO;2-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.10.011"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/00498254.2010.530700"},{"key":"e_1_2_7_6_1","first-page":"392","article-title":"Overview of drugs used for epilepsy and seizures etiology, diagnosis, and treatment","volume":"35","author":"Goldenberg MM","year":"2010","journal-title":"Pharmacy and Therapeutics"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.04.021"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx700079z"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2006.07.017"},{"key":"e_1_2_7_10_1","unstructured":"International Conference on Harmonization.Harmonized Tripartite Guideline: Validation of Analytical procedures: Text and Methodology Q2 (R1) 2005."},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(00)00013-9"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13318-012-0098-5"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2013.03.062"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfl096"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02307308"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1191\/096032700678815648"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425250903329095"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(01)00217-X"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.30.7.831"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24229"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2012.11.026"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2012.01.020"},{"key":"e_1_2_7_23_1","first-page":"644","article-title":"Bioanalytical method validation of valproic acid in human plasma in\u2010vitro after derivatization with 2,4\u2010dibromoacetophenon by high performance liquid chromatography\u2013photo diode array and its application to in\u2010vivo study","volume":"4","author":"Rahayu ST","year":"2013","journal-title":"International Journal of Pharmacy Teaching and Practices"},{"volume-title":"Guidance for Industry: Bioanalytical Method Validation","year":"2001","author":"US Food and Drug Administration","key":"e_1_2_7_24_1"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3109\/00498250903568504"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.01.018"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.04.002"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3307","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.3307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:27:04Z","timestamp":1760045224000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.3307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,19]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1002\/bmc.3307"],"URL":"https:\/\/doi.org\/10.1002\/bmc.3307","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2014,8,19]]}}}