{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T23:42:25Z","timestamp":1760053345396,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T00:00:00Z","timestamp":1411948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Program for Science and Technology Research of Hunan Province","award":["2012WK3044"],"award-info":[{"award-number":["2012WK3044"]}]},{"name":"the Health Department Foundation of Hunan Province","award":["132013-028"],"award-info":[{"award-number":["132013-028"]}]},{"name":"the National Science and Technology Major Projects","award":["2012ZX09303014-001"],"award-info":[{"award-number":["2012ZX09303014-001"]}]},{"name":"the National Scientif ic Foundation of China","award":["81373476"],"award-info":[{"award-number":["81373476"]}]},{"name":"the National Scientif ic Foundation of China","award":["81302851"],"award-info":[{"award-number":["81302851"]}]},{"name":"the National Scientif ic Foundation of China","award":["81301924"],"award-info":[{"award-number":["81301924"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dimemorfan phosphate has been widely used for 40\u2009years throughout the world for the treatment of coughs. This is the first report on the use of an LC\u2010MS\/MS\u2010based assay for the determination of dimemorfan in human plasma using estazolam as an internal standard after one\u2010step protein precipitation with acetonitrile. Chromatographic separation was achieved on a Phenomenex Luna C<jats:sub>18<\/jats:sub> column (3\u2009\u00b5m, 50\u2009\u00d7\u20092.0\u2009mm) using a fast gradient method, which involves water and methanol as the mobile phase (both containing 0.1% formic acid). Dimemorfan and estazolam were detected with proton adducts at <jats:italic>m\/z<\/jats:italic> values of 255.8\u2009\u2192\u2009155.1 and 295.0\u2009\u2192\u2009267.0, respectively, in the selected reaction monitoring positive mode. The linear dynamic range of the assay was 0.04\u20135.00\u2009ng\/mL. The chromatographic run time for each plasma sample was &lt;5\u2009min. The method was proven to be accurate, precise, and repeatable. Finally, the developed method was successfully applied for the determination of dimemorfan in a pharmacokinetic study using healthy Chinese subjects. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3325","type":"journal-article","created":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T03:33:09Z","timestamp":1411961589000},"page":"647-653","source":"Crossref","is-referenced-by-count":0,"title":["Simple and sensitive LC\u2010MS\/MS\u2010based assay for the quantification of dimemorfan in human plasma for use in a pharmacokinetic study"],"prefix":"10.1002","volume":"29","author":[{"given":"Hongyi","family":"Tan","sequence":"first","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinfu","family":"Peng","sequence":"additional","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"},{"name":"School of Pharmaceutical Sciences Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Pei","sequence":"additional","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liu","family":"Yang","sequence":"additional","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"},{"name":"School of Pharmaceutical Sciences Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Chen","sequence":"additional","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengxian","family":"Guo","sequence":"additional","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Hua","sequence":"additional","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Yuan","sequence":"additional","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoping","family":"Yang","sequence":"additional","affiliation":[{"name":"Center of Clinical Pharmacology, Third Xiangya Hospital Central South University  Changsha Hunan 410013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,29]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"China Food and Drug Administration.Technical Guidelines: Human Bioavailability and Bioequivalence Study of Chemico\u2010pharmaceutical Preparations 2005. 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