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To determine MDA level in various biological samples (human plasma, fish liver tissue and cells in culture), we used an HPLC method with fluorescent detection based on 2\u2010thiobarbituric acid (TBA) assay. The method was validated by the use of spiked pooled plasma samples. In tested concentration range (0.15\u20133.0\u2009\u00b5mol\/L) the method was linear (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.9963), the between\u2010day variability (coefficient of variations, CVs) was between 4.7 and 7.6%, the within\u2010day variability CVs was between 2.6 and 6.4% and recovery was between 91.2 and 107.6%. The level of MDA in human plasma (healthy male, non\u2010smokers, 46.3\u2009\u00b1\u20094.7\u2009years; <jats:italic>N<\/jats:italic>\u2009=\u200938) was 2.2\u2009\u00b1\u20091.4\u2009\u00b5mol\/L; that in liver tissue of common carp (<jats:italic>Cyprinus carpio<\/jats:italic>; <jats:italic>N<\/jats:italic>\u2009=\u200912) was 0.02\u2009\u00b1\u20090.004\u2009\u00b5mol\/g tissue, and in cultured cells (human laryngeal carcinoma cells; <jats:italic>N<\/jats:italic>\u2009=\u200910) it was 0.18\u2009\u00b1\u20090.02\u2009nmol\/mg proteins. The HPLC\u2010FL method is rapid, accurate and reliable to follow the extent of LPO in various biological samples, particularly in samples in which a low level of MDA is expected, such as cells in culture. Owing to the rapid analytical process and run time, it can be used for routine analysis of MDA in clinical laboratory. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3361","type":"journal-article","created":{"date-parts":[[2014,10,30]],"date-time":"2014-10-30T02:11:36Z","timestamp":1414635096000},"page":"41-46","source":"Crossref","is-referenced-by-count":92,"title":["Quantification of malondialdehyde by HPLC\u2010FL \u2013 application to various biological samples"],"prefix":"10.1002","volume":"29","author":[{"given":"Ana\u2010Marija","family":"Domijan","sequence":"first","affiliation":[{"name":"University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovica","family":"Rali\u0107","sequence":"additional","affiliation":[{"name":"Galapagos Research Center  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Radi\u0107 Brkanac","sequence":"additional","affiliation":[{"name":"University of Zagreb Faculty of 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