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The work presented here involves the synthesis and application of terpolymeric sorbent with different functionalizations adopted for the selective enrichment of biomolecules of interest from biological fluids. Synthesis of terpolymer was carried out by the radical polymerization of monomers: methyl acrylate, acrylic acid and vinyl acetate with diethylene glycol dimethacrylate as cross\u2010linking agent, benzoyl peroxide as initiator and chloroform as a porogenic solvent. Characterization was done through Fourier transform infrared spectroscopy, scanning electron microscopy and nitrogen adsorption porosimetry. The polymer was further modified to immobilized metal ion affinity chromatographic material, with immobilized Fe<jats:sup>3+<\/jats:sup>\/La<jats:sup>3+<\/jats:sup> ions that allowed phosphopeptide enrichment from tryptic digests of standard proteins as well as milk, egg yolk and human serum. Sensitivity of enrichment down to 50 fmol was achieved in the presence of complex protein background as bovine serum albumin. Hydrophobicity was introduced through octadecyl amine, which provides comparable results to ZipTip C<jats:sub>18<\/jats:sub>\/C<jats:sub>4<\/jats:sub> for desalting of complex mixtures of all caseins. Analysis of the enriched content was performed by Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI\u2010MS). Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3382","type":"journal-article","created":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T03:52:09Z","timestamp":1416196329000},"page":"981-989","source":"Crossref","is-referenced-by-count":6,"title":["Development of new multifunctional terpolymer sorbent for proteomics applications"],"prefix":"10.1002","volume":"29","author":[{"given":"Muhammad","family":"Najam\u2010ul\u2010Haq","sequence":"first","affiliation":[{"name":"Institute of Chemical Sciences Bahauddin Zakariya University  Multan 60800 Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adeela","family":"Saeed","sequence":"additional","affiliation":[{"name":"Institute of Chemical Sciences Bahauddin Zakariya University  Multan 60800 Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahmida","family":"Jabeen","sequence":"additional","affiliation":[{"name":"Institute of Chemical Sciences Bahauddin 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