{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T07:04:10Z","timestamp":1773903850983,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,1,23]],"date-time":"2015-01-23T00:00:00Z","timestamp":1421971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81171417"],"award-info":[{"award-number":["81171417"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A novel molecularly imprinted polymer (MIP) monolith for highly selective extraction of cholecystokinin (CCK) neuropeptides was prepared in a micropipette tip. The MIPs were synthesized by epitope imprinting technique and the polymerization conditions were investigated and optimized. The synthesized MIPs were characterized by infrared spectroscopy, elemental analyzer and scanning electron microscope. A molecularly imprinted solid\u2010phase microextraction (MI\u2010<jats:italic>\u03bc<\/jats:italic>\u2010SPE) method was developed for the extraction of CCK neuropeptides in aqueous solutions. The parameters affecting MI\u2010<jats:italic>\u03bc<\/jats:italic>\u2010SPE were optimized. The results indicated that this MIP monolith exhibited specific recognition capability and high enrichment efficiency for CCK neuropeptides. In addition, it showed excellent reusability. This MIP monolith was used for desalting and enrichment of CCK4, CCK5 and CCK8 from human cerebrospinal fluid prior to matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry analysis, and the results show that this MIP monolith can be a useful tool for effective purification and highly selective enrichment of multiple homologous CCK neuropeptides in cerebrospinal fluid simultaneously. By employing MI\u2010<jats:italic>\u03bc<\/jats:italic>\u2010SPE combined with HPLC\u2010ESI\u2010MS\/MS analysis, endogenous CCK4 in human cerebrospinal fluid was quantified. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3418","type":"journal-article","created":{"date-parts":[[2015,1,23]],"date-time":"2015-01-23T06:22:02Z","timestamp":1421994122000},"page":"1280-1289","source":"Crossref","is-referenced-by-count":20,"title":["Preparation and application of a novel molecularly imprinted solid\u2010phase microextraction monolith for selective enrichment of cholecystokinin neuropeptides in human cerebrospinal fluid"],"prefix":"10.1002","volume":"29","author":[{"given":"Xiang","family":"Ji","sequence":"first","affiliation":[{"name":"College of Chemistry and Molecular Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chemistry and Molecular Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chemistry and Molecular Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,1,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00599.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2012.03.004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.11.91"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-010-0399-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-0115(96)02104-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.08.022"},{"key":"e_1_2_7_8_1","first-page":"619","article-title":"Neuropeptide systems and schizophrenia. 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