{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:50:05Z","timestamp":1760061005181,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,2,24]],"date-time":"2015-02-24T00:00:00Z","timestamp":1424736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, sensitive, selective and precise high\u2010performance thin\u2010layer chromatographic method was developed for determination of lipid A (MPLA) adjuvant as a bulk and in solid fat nanoemulsions. Chromatographic separations were performed on thin\u2010layer chromatography aluminum plates precoated with silica gel 60\u2009F\u2010254 as stationary phase and chloroform\u2013methanol\u2013ethyl acetate solution (10:2:4, v\/v\/v) as mobile phase. With this solvent system, compact spots for MPLA at <jats:italic>R<\/jats:italic><jats:sub>f<\/jats:sub> value 0.80\u2009\u00b1\u20090.02 were obtained. Densitometric analysis of MPLA was carried out in absorbance mode at 357\u2009nm. Linear regression analysis for the calibration plots showed good linear relationship with <jats:italic>r<\/jats:italic>\u2009=\u20090.9996 in the concentration range of 20\u2013100\u2009ng\/spot. The mean values (\u00b1SD) of slope and intercept were found to be 7.355\u2009\u00b1\u20090.006 and 109.52\u2009\u00b1\u20090.170, respectively. Limits of detection (LOD) and quantitation (LOQ) were observed at 3.096 and 9.382\u2009ng\/spot, respectively.The method was validated for precision, accuracy, robustness and recovery as per the International Conference on Harmonization guidelines. Statistical analysis proved that the developed method for quantification of MPLA as a bulk and in solid fat nanoemulsions is reproducible, selective and economical. This method could be applied for quantitative assay of MPLA in lipid\u2010based vaccine formulations. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3444","type":"journal-article","created":{"date-parts":[[2015,2,24]],"date-time":"2015-02-24T02:07:12Z","timestamp":1424743632000},"page":"1473-1479","source":"Crossref","is-referenced-by-count":9,"title":["Development and validation of TLC\u2010densitometric method for determination of lipid A adjuvant as a bulk and in solid fat nanoemulsions"],"prefix":"10.1002","volume":"29","author":[{"given":"Sunita","family":"Minz","sequence":"first","affiliation":[{"name":"SLT Institute of Pharmaceutical sciences Guru Ghasidas Vishwavidyalaya  Bilaspur C.G. 495009 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Kaurav","sequence":"additional","affiliation":[{"name":"SLT Institute of Pharmaceutical sciences Guru Ghasidas Vishwavidyalaya  Bilaspur C.G. 495009 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kantrol Kumar","family":"Sahu","sequence":"additional","affiliation":[{"name":"SLT Institute of Pharmaceutical sciences Guru Ghasidas Vishwavidyalaya  Bilaspur C.G. 495009 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivekananda","family":"Mandal","sequence":"additional","affiliation":[{"name":"SLT Institute of Pharmaceutical sciences Guru Ghasidas Vishwavidyalaya  Bilaspur C.G. 495009 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravi Shankar","family":"Pandey","sequence":"additional","affiliation":[{"name":"SLT Institute of Pharmaceutical sciences Guru Ghasidas Vishwavidyalaya  Bilaspur C.G. 495009 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2009.08.022"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2005.03.021"},{"key":"e_1_2_8_4_1","unstructured":"AnselemS LowellGH AvivHandFriedmanD.Solid fat nanoemulsions as vaccine delivery vehicles. 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