{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T01:00:43Z","timestamp":1770512443877,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,3,22]],"date-time":"2015-03-22T00:00:00Z","timestamp":1426982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"East Tennessee State University Research Development Committee","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Opioid use during pregnancy can result in the newborn being physically dependent on the substance, thus experiencing drug withdrawal, termed neonatal abstinence syndrome (NAS). Buprenorphine and methadone are two drugs used to treat opioid withdrawal and are approved for use in pregnancy. Quantification of these compounds in umbilical cord plasma would help assess <jats:italic>in utero<\/jats:italic> exposure of neonates in cases of buprenorphine or methadone use during pregnancy. An LC\u2010MS\/MS method using solid\u2010phase extraction sample preparation was developed and validated for the simultaneous quantification of methadone, buprenorphine, norbuprenorphine, and glucuronide metabolites in umbilical cord plasma. The average accuracy (percentage error) and precision (relative standard deviation) were &lt;15% for each validated concentration. Our data establishes a 2 week maximum freezer storage window in order to achieve the most accurate cord plasma concentrations of these analytes. Additionally, we found that the umbilical cord tissue analysis was less sensitive compared with analysis with umbilical cord blood plasma, indicating that this may be a more appropriate matrix for determination of buprenorphine and metabolite concentrations. This method was successfully applied to the analysis of cord blood from women with known buprenorphine or methadone use during pregnancy. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3460","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T14:03:58Z","timestamp":1427205838000},"page":"1567-1574","source":"Crossref","is-referenced-by-count":14,"title":["UHPLC\u2010MS\/MS quantification of buprenorphine, norbuprenorphine, methadone, and glucuronide conjugates in umbilical cord plasma"],"prefix":"10.1002","volume":"29","author":[{"given":"Amy Redmond","family":"Kyle","sequence":"first","affiliation":[{"name":"Bill Gatton College of Pharmacy East Tennessee State University  Johnson City TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Carmical","sequence":"additional","affiliation":[{"name":"Bill Gatton College of Pharmacy East Tennessee State University  Johnson City TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darshan","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Quillen College of Medicine East Tennessee State University  Johnson City TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Pryor","sequence":"additional","affiliation":[{"name":"Vanderbilt University Medical Center\/Monroe Carell Children's Hospital Neonatal Perinatal Medicine  Nashville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacy","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy East Tennessee State University  Johnson City TN 37614\u20101708 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19990630)13:12<1175::AID-RCM639>3.0.CO;2-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/09595230701494416"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.07.028"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(03)00169-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2009.04.005"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-007-1228-9"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2004.11.013"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.113712"},{"key":"e_1_2_7_10_1","first-page":"e1","article-title":"Current trends in narcotic use in pregnancy and neonatal outcomes","volume":"259","author":"Kellogg A","year":"2011","journal-title":"American Journal of Obstetrics and Gynecology"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.02.020"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2007.12.017"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.12.023"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.09.035"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.300.1.26"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2004.09.008"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2009.11.015"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.11.025"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2012.07.001"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cct.2014.08.009"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2005.04.012"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3460","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.3460","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:55:56Z","timestamp":1760039756000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.3460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,22]]},"references-count":20,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1002\/bmc.3460"],"URL":"https:\/\/doi.org\/10.1002\/bmc.3460","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,22]]}}}