{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:50:25Z","timestamp":1760061025210,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,4,6]],"date-time":"2015-04-06T00:00:00Z","timestamp":1428278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["21075097"],"award-info":[{"award-number":["21075097"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monosialotetrahexosylganglioside (GM1), one of glycosphingolipids containing sialic acid, plays particularly important role in fighting against paralysis, dementia and other diseases caused by brain and nerve damage. In this work, a simple and highly efficient method with high yield was developed for isolation and purification of GM1 from pig brain. The method consisted of an extraction by chloroform\u2013methanol\u2013water and a two\u2010step chromatographic separation by DEAE\u2013Sepharose Fast Flow anion\u2010exchange medium and Sephacryl S\u2010100 HR size\u2010exclusion medium. The purified GM1 was proved to be homogeneous and had a purity of &gt;98.0% by high\u2010performance anion\u2010exchange and size\u2010exclusion chromatography. The molecular weight was 30.0\u2009kDa by high\u2010performance size\u2010exclusion chromatography and 1546.9\u2009Da by electrospray ionization mass spectrometry. The chromogenic reaction by resorcinol\u2013hydrochloric acid solution indicated that the purified GM1 showed a specific chromogenic reaction of sialic acid. Through this isolation and purification program, ~1.0\u2009mg of pure GM1 could be captured from 500\u2009g wet pig brain tissue and the yield of GM1 was around 0.022%, which was higher than the yields by other methods. The method may provide an alternative for isolation and purification of GM<jats:sub>1<\/jats:sub> in other biological tissues. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3467","type":"journal-article","created":{"date-parts":[[2015,4,6]],"date-time":"2015-04-06T23:56:58Z","timestamp":1428364618000},"page":"1604-1611","source":"Crossref","is-referenced-by-count":4,"title":["Isolation and purification of monosialotetrahexosylgangliosides from pig brain by extraction and liquid chromatography"],"prefix":"10.1002","volume":"29","author":[{"given":"Liujiao","family":"Bian","sequence":"first","affiliation":[{"name":"College of Life Science Northwest University  Xi'an 710069 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianting","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Science Northwest University  Xi'an 710069 China"},{"name":"Weapon Industry 521 Hospital  Xi'an 710065 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"College of 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