{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:47:37Z","timestamp":1760060857894,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T00:00:00Z","timestamp":1432598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U10 HD40521","U10 HD46000","UL1 TR42"],"award-info":[{"award-number":["U10 HD40521","U10 HD46000","UL1 TR42"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inositol is a six\u2010carbon sugar alcohol and is one of nine biologically significant isomers of hexahydroxycyclohexane. <jats:italic>Myo<\/jats:italic>\u2010inositol is the primary biologically active form and is present in higher concentrations in the fetus and newborn than in adults. It is currently being examined for the prevention of retinopathy of prematurity in newborn preterm infants. A robust method for quantifying <jats:italic>myo<\/jats:italic>\u2010inositol (MI), <jats:sc>D<\/jats:sc>\u2010<jats:italic>chiro<\/jats:italic>\u2010inositol (DCI) and 1,5\u2010anhydro\u2010 <jats:sc>D<\/jats:sc>\u2010sorbitol (ADS) in very small\u2010volume (25 \u03bcL) urine, blood serum and\/or plasma samples was developed. Using a multiple\u2010column, multiple mobile phase liquid chromatographic system with electrochemical detection, the method was validated with respect to (a) selectivity, (b) accuracy\/recovery, (c) precision\/reproducibility, (d) sensitivity, (e) stability and (f) ruggedness. The standard curve was linear and ranged from 0.5 to 30 mg\/L for each of the three analytes. Above\u2010mentioned performance measures were within acceptable limits described in the Food and Drug Administration's <jats:italic>Guidance for Industry<\/jats:italic>: <jats:italic>Bioanalytical Method Validation<\/jats:italic>. The method was validated using blood serum and plasma collected using four common anticoagulants, and also by quantifying the accuracy and sensitivity of MI measured in simulated urine samples recovered from preterm infant diaper systems. The method performs satisfactorily measuring the three most common inositol isomers on 25 \u03bcL clinical samples of serum, plasma, milk, and\/or urine. Similar performance is seen testing larger volume samples of infant formulas and infant formula ingredients. MI, ADS and DCI may be accurately tested in urine samples collected from five different preterm infant diapers if the urine volume is greater than 2\u20135 mL. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3470","type":"journal-article","created":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T04:22:21Z","timestamp":1432614141000},"page":"1629-1636","source":"Crossref","is-referenced-by-count":10,"title":["Quantification of <i>myo<\/i>\u2010inositol, 1,5\u2010anhydro\u2010 <scp>D<\/scp>\u2010sorbitol, and <scp>D<\/scp>\u2010chiro\u2010inositol using high\u2010performance liquid chromatography with electrochemical detection in very small volume clinical samples"],"prefix":"10.1002","volume":"29","author":[{"given":"Karen J.","family":"Schimpf","sequence":"first","affiliation":[{"name":"Abbott Nutrition  3300 Stelzer Road Columbus OH 34219 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia C.","family":"Meek","sequence":"additional","affiliation":[{"name":"School of Pharmacy Texas Tech University Health Sciences Center  5920 Forest Park Road \u2013 Suite 400 Dallas TX 75235 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