{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:48:17Z","timestamp":1760060897944,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,4,16]],"date-time":"2015-04-16T00:00:00Z","timestamp":1429142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81173009","81302722"],"award-info":[{"award-number":["81173009","81302722"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Scientific Research Foundation","award":["QNJJ2013504"],"award-info":[{"award-number":["QNJJ2013504"]}]},{"name":"Special Scientific Research Foundation in Shenyang Pharmaceutical University","award":["ZCJJ2014409"],"award-info":[{"award-number":["ZCJJ2014409"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A sensitive, selective and high\u2010throughput UPLC\u2010MS\/MS method was developed and validated for the determination of a novel c\u2010Met tyrosine kinase inhibitor, QBH\u2010196, in rat plasma. QBH\u2010196 and its analog BH357 (IS) were extracted from rat plasma using a mixture of dichloromethane and <jats:italic>N<\/jats:italic>\u2010hexane (2:3, v\/v). The chromatographic separation was carried out on Phenomenex C<jats:sub>18<\/jats:sub> column (50 \u00d7 2.1 mm, 2.6 \u00b5m particle size) with a gradient mobile phase of methanol (A) and water containing 0.05% formic acid (B) at a flow rate of 0.2 mL\/min. The assay was performed by positive electrospray ionization in multiple reaction monitoring mode using transitions of <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 622.68 \u2192 140.41 for QBH\u2010196 and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 591.19 \u2192126.21 for the IS, respectively. Good linearity was obtained over the concentration range of 8.0\u20134000 ng\/mL (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> &gt; 0.99) for QBH\u2010196 and the lower limit of quantification was 8.0 ng\/mL in rat plasma. Validations of the method, including its sensitivity, extraction recovery, matrix effect, intra\u2010 and inter\u2010day precision, accuracy and stability, were all within acceptable limits. The established method was successfully applied to determine absolute oral bioavailability of QBH\u2010196 in rats for the first time. The mean oral absolute bioavailability of QBH\u2010196 was found to be about 40.8% and the elimination half\u2010life was 40.0 \u00b1 13.1 h. This result suggested that QBH\u2010196 exhibits good oral absorption <jats:italic>in vivo<\/jats:italic>, which is very important for the further development of QBH\u2010196 as a new oral anticancer drug. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3474","type":"journal-article","created":{"date-parts":[[2015,4,16]],"date-time":"2015-04-16T03:25:10Z","timestamp":1429154710000},"page":"1650-1656","source":"Crossref","is-referenced-by-count":4,"title":["A rapid and sensitive LC\u2010MS\/MS method for evaluation of the absolute oral bioavailability of a novel c\u2010Met tyrosine kinase inhibitor QBH\u2010196 in rats"],"prefix":"10.1002","volume":"29","author":[{"given":"Qi.","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Biopharmaceutics, School of Pharmacy Shenyang Pharmaceutical University  No. 103, Wenhua Road Shenyang 110016 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Biopharmaceutics, School of Pharmacy Shenyang Pharmaceutical University  No. 103, Wenhua Road Shenyang 110016 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