{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:49:03Z","timestamp":1760060943826,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,6,16]],"date-time":"2015-06-16T00:00:00Z","timestamp":1434412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dried blood spot analysis is an innovative novel blood sampling technique gaining interest in drug discovery and development processes owing to its inherent advantages over the conventional whole blood, plasma or serum sample collection. The present manuscript describes the development and validation of a highly sensitive and precise method of evaluation of pharmacokinetics of (+) and (\u2212) darunavir enantiomers on rat dried blood spots. The enantiomers on rat dried blood spots were extracted into methanol and separated by LC on a Chiralpak IA column using hexane and ethanol containing 0.1% DEA (75:25, v\/v) as a mobile phase at 20\u00b0C; both the enantiomers were detected at 266 nm using a photodiode array detector. The method was validated in terms of selectivity, linearity, accuracy, precision and stability as per the US Food and Drug and Administration guidelines. The hematocrit effect on extraction recovery was evaluated and the mean recoveries of (\u2212) and (+) enantiomers of darunavir from dried blood spots were found to be 85.76 and 88.91% respectively. The intra\u2010 and inter\u2010day precision and accuracy were 3.1\u20138.4 and 0.8\u20134.8% respectively. The developed method was successfully applied to a pharmacokinetic study of (+) and (\u2212) enantiomers of darunavir on rat dried blood spots. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3510","type":"journal-article","created":{"date-parts":[[2015,6,16]],"date-time":"2015-06-16T22:23:32Z","timestamp":1434493412000},"page":"1878-1884","source":"Crossref","is-referenced-by-count":3,"title":["Dried blood spot analysis of (+) and (\u2212) darunavir enantiomers on immobilized amylose tris\u2010(3, 5\u2010dimethylphenylcarbamate) LC and its application to pharmacokinetics"],"prefix":"10.1002","volume":"29","author":[{"given":"Nageswara Rao","family":"Ramisetti","sequence":"first","affiliation":[{"name":"Analytical Chemistry Division, Discovery Laboratory Indian Institute of Chemical Technology  Tarnaka Hyderabad 500 607 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manikanta Swamy","family":"Arnipalli","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Division, Discovery Laboratory Indian Institute of Chemical Technology  Tarnaka Hyderabad 500 607 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narendra Varma","family":"Nimmu","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Division, Discovery Laboratory Indian Institute of Chemical Technology  Tarnaka Hyderabad 500 607 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,16]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1","article-title":"Development and validation of infrared spectroscopy method for the determination of darunavir in tablets","volume":"3","author":"Ana Carolina K","year":"2013","journal-title":"Journal of Physical Chemistry"},{"volume-title":"The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals","year":"2001","author":"Budavari 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