{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:49:02Z","timestamp":1781246942666,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Astragaloside III (AST III), a naturally occurring saponin compound isolated from Radix Astragali, has been demonstrated to have anti\u2010gastric ulcer, immunomodulatory and antitumor effects. To evaluate its pharmacokinetics in rats, a rapid, sensitive and specific high\u2010performance liquid chromatography\u2013tandem mass spectrometric (HPLC\u2010MS\/MS) method has been developed and validated for the quantification of astragaloside III in rat plasma. Samples were pretreated using a simple protein precipitation with methanol\u2013acetonitrile (50:50, v\/v) and the chromatographic separation was performed on a C<jats:sub>18<\/jats:sub> column by a gradient elution using a mobile phase consisting of water containing 0.1% formic acid and acetonitrile containing 0.1% formic acid. Astragaloside III and the internal standard (buspirone) were detected using a tandem mass spectrometer in positive multiple reaction monitoring mode. Method validation revealed excellent linearity over the range of 5.00\u20135000 ng\/mL together with satisfactory intra\u2010 and inter\u2010day precision, accuracy and recovery. Stability testing showed that astragaloside III spiked into rat plasma was stable for 24 h at 20\u00b0C temperature, for up to 30 days at \u221280\u00b0C, and during three freeze\u2013thaw cycles. The method was successfully used to investigate the pharmacokinetic profile of AST III after oral (10 mg\/kg) and intravenous (1.0 mg\/kg) administration in rats. The oral absolute bioavailability of AST III was calculated to be 4.15 \u00b1 0.67% with an elimination half\u2010life value of 2.13 \u00b1 0.11 h, suggesting its poor absorption and\/or strong metabolism <jats:italic>in vivo<\/jats:italic>. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3521","type":"journal-article","created":{"date-parts":[[2015,5,29]],"date-time":"2015-05-29T12:33:28Z","timestamp":1432902808000},"page":"105-110","source":"Crossref","is-referenced-by-count":9,"title":["Determination of astragaloside III in rat plasma by liquid chromatography\u2013tandem mass spectrometry and its application to a rat pharmacokinetic study"],"prefix":"10.1002","volume":"30","author":[{"given":"Yongsong","family":"Zhai","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine Capital Medical University  Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyue","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing 100700 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine Capital Medical University  Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muxin","family":"Gong","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine Capital Medical University  Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Qiu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine Capital Medical University  Beijing 100069 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1577","article-title":"Phenolic derivatives from Radix Astragali and their anti\u2010inflammatory activities","volume":"9","author":"Chen W","year":"2014","journal-title":"Natural Products Communication"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/36.3.211"},{"key":"e_1_2_6_4_1","unstructured":"European Medicines Agency.Guideline on Bioanalytical Method Validation EMEA\/CHMP\/EWP\/192217\/2009. 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