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Alprenolol was applied as an internal standard. A BEH C<jats:sub>18<\/jats:sub> (2.1 \u00d7 50 mm, 1.7 \u00b5m) column provided chromatographic separation of analytes using a binary mobile phase gradient (A, 2 mmol\/L ammonium acetate, 0.1% formic acid in 5% acetonitrile, v\/v\/v; B, 2 mmol\/L ammonium acetate, 0.1% formic acid in 95% acetonitrile, v\/v\/v). Mass spectrometric detection was performed in the positive electrospray ionization mode and ion suppression owing to matrix effects was evaluated. The validation criteria were determined: linearity, precision, accuracy, recovery, limit of detection, limit of quantification, reproducibility and matrix effect. The concentration range was as follows: 0.25\u20131000 ng\/mL for agomelatine; 0.25\u2013100 ng\/mL for asenapine and iloperidone; 2.5\u20131000 ng\/mL for amisulpride, aripiprazole, vilazodone and zotepine; 2.3\u2013924.6 ng\/mL for dehydroaripiprazole; 2.2\u2013878.4 ng\/mL for melperone; and 2.2\u2013883.5 ng\/mL for ziprasidone. Limits of quantitation below a therapeutic reference range were achieved for all analytes. Intra\u2010run precision of 0.4\u20135.5 %, inter\u2010run precision of 0.6\u20138.2% and overall recovery of 87.9\u2013114.1% were obtained. The validated method was successfully implemented into routine practice for therapeutic drug monitoring in our hospital. 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