{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:20:59Z","timestamp":1761513659407,"version":"build-2065373602"},"reference-count":11,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,8,6]],"date-time":"2015-08-06T00:00:00Z","timestamp":1438819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A novel, sensitive and selective ultra\u2010high\u2010performance liquid chromatography\u2013electrospray ionization mass spectrometry method was developed and validated for the quantification of acotiamide (ACT), a first\u2010in\u2010class drug used in functional dyspepsia, in rat plasma. A simple protein precipitation method with acetonitrile as precipitating solvent was used to extract ACT from rat plasma. ACT and an internal standard (mirabegron, IS) were separated on an Agilent poroshell EC C<jats:sub>18<\/jats:sub> column (50 \u00d7 3.0\u2009mm, 2.7\u2009\u00b5m) using methanol\u201310\u2009mM ammonium acetate binary gradient mobile phase at a flow rate of 0.4\u2009mL\/min over 4\u2009min run time. Detection was performed using target ions of [M + H]<jats:sup>+<\/jats:sup> at <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 451.2010 for ACT and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 397.1693 for IS in selective ion mode. The method was validated in the calibration range of 1.31\u20131000\u2009ng\/mL. All the validation parameters were well within the limits. The method demonstrated good performances in terms of intra\u2010 and inter\u2010day precision (3.27\u201312.60% CV) and accuracy (87.96\u2013104.94%). Thus the present ultra\u2010high\u2010pressure liquid chromatograhy\u2013high\u2010resolution mass spectrometry method for determination of ACT in rat plasma, is highly sensitive and rapid with a short run\u2010time of 4\u2009min, can be suitable for high sample throughput and for large batches of biological samples in pharmacokinetic studies. This method can be extended to measure plasma concentrations of ACT in humans to understand drug metabolism, drug interaction and adverse effects. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3555","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T23:08:50Z","timestamp":1436310530000},"page":"363-368","source":"Crossref","is-referenced-by-count":10,"title":["Quantitation of acotiamide in rat plasma by UHPLC\u2010Q\u2010TOF\u2010MS: method development, validation and application to pharmacokinetics"],"prefix":"10.1002","volume":"30","author":[{"given":"Prinesh N.","family":"Patel","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Balanagar Hyderabad 500037 Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradipbhai D.","family":"Kalariya","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Balanagar Hyderabad 500037 Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Challa Veerabhadra","family":"Swamy","sequence":"additional","affiliation":[{"name":"Department of Pharmacology National Institute of Pharmaceutical Education and Research  Balanagar Hyderabad 500037 Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Gananadhamu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Balanagar Hyderabad 500037 Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Srinivas","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Balanagar Hyderabad 500037 Telangana India"},{"name":"National Centre for Mass Spectrometry CSIR \u2013 Indian Institute of Chemical Technology  Hyderabad 500 007 Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,8,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.10.008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6439"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.08.025"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(00)00593-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/828719"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-013-0100-9"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.12.159"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01486.x"},{"key":"e_1_2_7_10_1","first-page":"124","article-title":"Food and Administration (FDA) guidance for industry: bioanalytical method validation. 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